Articles
EXPERIMENTS ON THE PRODUCTION OF VIRUS FREE RASPBERRY PROPAGATION MATERIAL BY MERISTEM CULTURE
Article number
95_9
Pages
77 – 78
Language
Abstract
Shoot tip meristem culture of raspberries was started with the purpose of eliminating heat stable viruses.
Shoot tips of stratified and forced raspberry roots were collected, disinfected in a chloramine solution, and meristem tips with 1–2 leaf primordia cut out and placed in an agargel medium containing macroelements of Morel, micro-elements of Heller, several B vitamins at 1 ppm, myoinositol 100 mg/l, glucose 2–3% and IBA at 0.1 ppm.
Rooting was induced using the same medium with a lowered auxin concentration.
Rooted plantlets could be dissected for further propagation or transplanted to soil.
Shoot tips of stratified and forced raspberry roots were collected, disinfected in a chloramine solution, and meristem tips with 1–2 leaf primordia cut out and placed in an agargel medium containing macroelements of Morel, micro-elements of Heller, several B vitamins at 1 ppm, myoinositol 100 mg/l, glucose 2–3% and IBA at 0.1 ppm.
Rooting was induced using the same medium with a lowered auxin concentration.
Rooted plantlets could be dissected for further propagation or transplanted to soil.
Authors
J. Vértesy
Keywords
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