Articles
THE DOUBLE EXPRESSION OF THE SF ALLELE IN ALMOND
Article number
967_13
Pages
121 – 125
Language
English
Abstract
The search for sources of almond self-compatibility (SC) other than the cultivars from the Italian region of Puglia was undertaken in order to avoid the problems derived from inbreeding depression in some offspring.
The Sf allele, considered to confer SC, was identified by specific primers in several genotypes which later on were shown to be self-incompatible (SI). Sequencing of this allele from both SC and SI genotypes showed their full genetic identity, not only in the coding region of both the Sf-RNase and the SFBf, but also in the 5 regulatory sequence of the Sf-RNase.
In addition, two RNase bands were obtained in the SI genotypes with the Sf allele as opposed to the production of a single RNase band in the SC genotypes.
Thus, the inactive Sf (Sfi) does not produce RNase and is linked to SC, whereas the active Sf (Sfa) is distinguished by producing RNase, as the other SI alleles, and is linked to SI. The genetic identity of both alleles is further confirmed by the recognition of the Sfi allele by the Sfa-RNase.
Consequently, the presence of the Sf gene is not the exclusive source of self-compatibility in almond and the reason for the different expression of the Sf is independent of the complete genetic identity in the whole chromosome region bordering the S locus.
Thus, other factors outside the S locus must be involved in the expression of almond self-compatibility.
The Sf allele, considered to confer SC, was identified by specific primers in several genotypes which later on were shown to be self-incompatible (SI). Sequencing of this allele from both SC and SI genotypes showed their full genetic identity, not only in the coding region of both the Sf-RNase and the SFBf, but also in the 5 regulatory sequence of the Sf-RNase.
In addition, two RNase bands were obtained in the SI genotypes with the Sf allele as opposed to the production of a single RNase band in the SC genotypes.
Thus, the inactive Sf (Sfi) does not produce RNase and is linked to SC, whereas the active Sf (Sfa) is distinguished by producing RNase, as the other SI alleles, and is linked to SI. The genetic identity of both alleles is further confirmed by the recognition of the Sfi allele by the Sfa-RNase.
Consequently, the presence of the Sf gene is not the exclusive source of self-compatibility in almond and the reason for the different expression of the Sf is independent of the complete genetic identity in the whole chromosome region bordering the S locus.
Thus, other factors outside the S locus must be involved in the expression of almond self-compatibility.
Authors
R. Socias i Company, O. Kodad, J.M. Alonso, A. Fernández i Martí
Keywords
Prunus amygdalus Batsch, self-compatibility, breeding, gene sequencing, modifier genes
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