YEAST TWO-HYBRID SCREENING FOR THE GENERAL INHIBITOR DETOXIFYING S-RNASE IN PRUNUS

Many species in the Rosaceae, Solananceae and Plantaginaceae exhibit the S-RNase-based self-incompatibility (SI) system.
In this system, pistil and pollen specificities are conferred by S-RNase and S locus F-box protein (SFB/SLF). In addition to these specificity determinants, other SI general factors or SI modifiers have been shown to be required for the SI reaction.
Although the hypothetical general inhibitor (GI) detoxifying S-RNase has been proposed to be present in Prunus, it is yet to be identified.
This study investigated E3-ligase like protein genes that were isolated by the yeast two hybrid assay (Y2H) and RT-PCR to test the possibility that they could be GI, since the ubiquitin proteasome system (UPS) was reported to be involved in S-RNase detoxification in the Solananceae and Plantaginaceae. A series of the Y2H and GST pull-down assays conducted in this study, however, did not detect any interactions between S-RNase and E3-ligase like proteins.

I Workshop on Floral Biology and S-Incompatibility in Fruit Species

D. Matsumoto, R. Tao

Prunus, self-incompatibility, S-RNase, yeast two-hybrid

https://doi.org/10.17660/ActaHortic.2012.967.19