Articles
THE RESPONSE OF GROWTH AND DEVELOPMENT FROM IN VITRO SEED PROPAGATION OF DENDROBIUM ORCHID TO CHITOSAN
Article number
970_18
Pages
173 – 176
Language
English
Abstract
Chitosan is a deacethylated derivative of chitin derived from the crustacean group.
Thus it can be degraded in nature and is safe for living organisms.
It has been shown to promote the growth of various plants.
For example, chitosan has been reported to increase the growth rate and early flowering in orchids.
The objective of this experiment was to study the effect of chitosan on in vitro growth of Dendrobium Queen Pink protocorms.
Protocorms at the approximate size of about 0.3-0.5 cm were cultured on modified Vacin and Went (VW) medium supplemented with chitosan at the concentrations of 10, 20, 40, 60 mg L-1 or without chitosan (control). The growths were recorded after being cultured for 1 year.
The results showed that seedlings grown on VW supplemented with chitosan at all concentrations had a higher fresh weight and dry weight when compared to the control.
Chitosan at the concentration of 60 mg L-1 had the highest plant height, root length and leaf area compared to other treatments including the control.
However, there was no statistical difference on the number of leaves, roots and new shoots.
The effect of chitosan on the number of stoma showed that chitosan at all concentrations increased the number of stomata in comparison to the control but the number and size of the chloroplasts was not different.
Thus it can be degraded in nature and is safe for living organisms.
It has been shown to promote the growth of various plants.
For example, chitosan has been reported to increase the growth rate and early flowering in orchids.
The objective of this experiment was to study the effect of chitosan on in vitro growth of Dendrobium Queen Pink protocorms.
Protocorms at the approximate size of about 0.3-0.5 cm were cultured on modified Vacin and Went (VW) medium supplemented with chitosan at the concentrations of 10, 20, 40, 60 mg L-1 or without chitosan (control). The growths were recorded after being cultured for 1 year.
The results showed that seedlings grown on VW supplemented with chitosan at all concentrations had a higher fresh weight and dry weight when compared to the control.
Chitosan at the concentration of 60 mg L-1 had the highest plant height, root length and leaf area compared to other treatments including the control.
However, there was no statistical difference on the number of leaves, roots and new shoots.
The effect of chitosan on the number of stoma showed that chitosan at all concentrations increased the number of stomata in comparison to the control but the number and size of the chloroplasts was not different.
Publication
Authors
K. Obsuwan, K. Sawangsri, A. Thongpukdee, C. Thepsithar
Keywords
chloroplast, fresh weight, dry weight, plant height, orchids, in vitro culture
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