Articles
BACILLUS MEGATERIUM ISOLATE 3103: ANTAGONISTIC SPECTRUM ON COLLETOTRICHUM GLOEOSPORIOIDES DIVERSITY AND IMPACT OF FIELD APPLICATION ON POSTHARVEST INCIDENCE OF MANGO FRUIT ANTHRACNOSE
Article number
973_9
Pages
81 – 88
Language
English
Abstract
Anthracnose, caused by Colletotrichum gloeosporioides, is one of the most serious postharvest diseases of mango (Mangifera indica) resulting from the quiescent phase of infection occurring in the field. Bacillus megaterium isolate 3103 (BM-3103) was the most outstanding among a large number of phylloplane bacterial flora tested in the laboratory for antagonism towards C. gloeosporioides. A mango-leaf assay was developed during 2007-2008 in order to evaluate the antagonistic potential of BM-3103 on C. gloeosporioides diversity.
The experiment was conducted on surface sterilized immature, light-green coloured leaves of Nam-dok-mai mango.
Drops of C. gloeosporioides spore suspensions, together with BM-3103 cell suspensions, were placed on the upper sides of the leaves at pre-marked target sites.
The inoculated leaves were incubated at 26-28°C in humid conditions to allow disease development.
Antagonism to 14 C. gloeosporioides isolates tested resulted in an average reduction in lesion diameter from 5.12 mm on non-protected to 0.79 mm on protected treatments, and proved that BM-3103 was an aggressive antagonist towards C. gloeosporioides diversity.
Biological control capability on quiescent infection caused by C. gloeosporioides was determined under commercial field conditions.
A BM-3103 cell suspension was sprayed on to Nam-dok-mai-seethong mango canopies every 14 days starting from flowering and continuing to harvesting of mature green fruit.
Incidence of anthracnose on postharvest mangoes incubated in conditions favouring disease development indicated promising efficacy of BM-3103 by the reduction in disease severity.
Diseased areas on the fruit surface, and total numbers of diseased fruit decreased by 20.3 and 13.5%, respectively 7 days after symptoms first appeared.
The experiment was conducted on surface sterilized immature, light-green coloured leaves of Nam-dok-mai mango.
Drops of C. gloeosporioides spore suspensions, together with BM-3103 cell suspensions, were placed on the upper sides of the leaves at pre-marked target sites.
The inoculated leaves were incubated at 26-28°C in humid conditions to allow disease development.
Antagonism to 14 C. gloeosporioides isolates tested resulted in an average reduction in lesion diameter from 5.12 mm on non-protected to 0.79 mm on protected treatments, and proved that BM-3103 was an aggressive antagonist towards C. gloeosporioides diversity.
Biological control capability on quiescent infection caused by C. gloeosporioides was determined under commercial field conditions.
A BM-3103 cell suspension was sprayed on to Nam-dok-mai-seethong mango canopies every 14 days starting from flowering and continuing to harvesting of mature green fruit.
Incidence of anthracnose on postharvest mangoes incubated in conditions favouring disease development indicated promising efficacy of BM-3103 by the reduction in disease severity.
Diseased areas on the fruit surface, and total numbers of diseased fruit decreased by 20.3 and 13.5%, respectively 7 days after symptoms first appeared.
Authors
U. Farungsang, S. Sinlapasunthorn, C. Rattanakreetakul , L. Phavaphutanon, N. Farungsang
Keywords
biological control, mango-leaf assay, Mangifera indica, phylloplane microorganism
Online Articles (32)