Articles
TRANSGENIC CARROT EXPRESSING THAUMATIN II GENE HAS ENHANCED RESISTANCE AGAINST FUSARIUM AVENACEUM
Article number
974_14
Pages
123 – 130
Language
English
Abstract
Transgenic carrot (Daucus carota L.) plants expressing a thaumatin II protein were obtained by Agrobacterium-mediated transformation.
Embryogenic calli of Nantskaya 4 were transformed by disarmed supervirulent agrobacterial strain CBE21 harboring the pBIThau35 plasmid.
The presence of the thauII gene was confirmed by PCR, and the expression of the thaumatin II protein in primary transgenic plants was confirmed by Western blot analysis.
Positive T0 carrot plants were self-pollinated and T1, T2 and T3 seeds were produced.
Resulted seeds were further selected for the antibiotic resistance and the inheritance and expression of thauII gene was evaluated by PCR and Western blot analysis.
From these, five groups of T2 and T3 progenies of two T0 lines were analyzed for the enhanced tolerance to the foliar and root pathogen Fusarium avenaceum. Among tested carrot plants, both T2 and T3 transgenic progenies of 40-61 line showed a fairly strong resistance against pathogen.
Embryogenic calli of Nantskaya 4 were transformed by disarmed supervirulent agrobacterial strain CBE21 harboring the pBIThau35 plasmid.
The presence of the thauII gene was confirmed by PCR, and the expression of the thaumatin II protein in primary transgenic plants was confirmed by Western blot analysis.
Positive T0 carrot plants were self-pollinated and T1, T2 and T3 seeds were produced.
Resulted seeds were further selected for the antibiotic resistance and the inheritance and expression of thauII gene was evaluated by PCR and Western blot analysis.
From these, five groups of T2 and T3 progenies of two T0 lines were analyzed for the enhanced tolerance to the foliar and root pathogen Fusarium avenaceum. Among tested carrot plants, both T2 and T3 transgenic progenies of 40-61 line showed a fairly strong resistance against pathogen.
Authors
T. Sidorova, D. Miroshnichenko, S. Dolgov, G. Tjukavin
Keywords
fungal resistance, pathogenesis-related protein, transgenic carrot
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