Articles
ALTERATION OF LEAF ANATOMY STRUCTURE IN MANGOSTEEN REGENERANTS IN VITRO CAUSED BY GAMMA RAY IRRADIATION
Article number
975_14
Pages
139 – 146
Language
English
Abstract
Mangosteen has a low of rate photosynthesis.
Research was conducted to study the alteration of leaf structure of mangosteen caused by gamma ray irradiation.
Leaf samples from 21 mutant regenerants and from a selected control were examined using both paradermal and transverse sections.
Paradermal sectioning was conducted by using a whole mount method followed by staining with safranin 1%, whereas transverse sections were prepared using paraffin methods where the leaf was sliced using a rotary microtome at 10 µm thickness and then stained with saffranin 1% and fastgreen 0.5%. Gamma ray irradiation altered leaf structure.
Several mutant regenerants had a higher stomata density and a higher stomatal index than control regenerants those regenerants were R-5/2, R-5/3, R-5/4, R-10/4, R-15/3, R-25/1,
R-30/1 and R-30/2. Transverse sections showed that the thickness of the upper cuticle of mutant regenerants was thinner than control regenerants, except for the mutant regenerant R-5/1. Regenerants R-10/4, R-15/1, R-15/2, R-15/3 had a thicker palisade parenchyma, spongy mesophyll and leaf lamina than control regenerants.
In addition, the spongy mesophyll was thicker and the number of vascular bundles was also greater than in the control regenerant.
Research was conducted to study the alteration of leaf structure of mangosteen caused by gamma ray irradiation.
Leaf samples from 21 mutant regenerants and from a selected control were examined using both paradermal and transverse sections.
Paradermal sectioning was conducted by using a whole mount method followed by staining with safranin 1%, whereas transverse sections were prepared using paraffin methods where the leaf was sliced using a rotary microtome at 10 µm thickness and then stained with saffranin 1% and fastgreen 0.5%. Gamma ray irradiation altered leaf structure.
Several mutant regenerants had a higher stomata density and a higher stomatal index than control regenerants those regenerants were R-5/2, R-5/3, R-5/4, R-10/4, R-15/3, R-25/1,
R-30/1 and R-30/2. Transverse sections showed that the thickness of the upper cuticle of mutant regenerants was thinner than control regenerants, except for the mutant regenerant R-5/1. Regenerants R-10/4, R-15/1, R-15/2, R-15/3 had a thicker palisade parenchyma, spongy mesophyll and leaf lamina than control regenerants.
In addition, the spongy mesophyll was thicker and the number of vascular bundles was also greater than in the control regenerant.
Authors
W.A. Qosim, R. Poerwanto, G.A. Wattimena, Witjaksono
Keywords
gamma ray irradiation, mutation, paradermal and transverse sections
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