Articles
DETECTING FUSARIUM OXYSPORUM F. SP. CUBENSE TROPICAL RACE 4 IN SOIL AND SYMPTOMLESS BANANA TISSUES
Article number
986_11
Pages
127 – 130
Language
English
Abstract
Tropical race 4 (TR4) of Fusarium oxysporum f. sp. cubense (Foc) is a quarantine pathogen in many banana-producing regions of the world.
Preventing further dissemination and precluding incursions into areas where it has not been observed is critical for maintaining local and commercial banana production.
Trading of symptomless but infected banana planting material, movement of machinery with adhering infested soil, and the use of traditional banana-based packing material facilitate the spread of Foc.
A PCR-based detection tool for TR4 was used to analyze field samples from symptomatic and symptomless banana plants and soils collected in TR4-affected areas.
Foc TR4 was detected in all infected samples by conventional or nested PCR analyses.
The results will be used to develop and implement TR4 quarantine and management strategies.
Preventing further dissemination and precluding incursions into areas where it has not been observed is critical for maintaining local and commercial banana production.
Trading of symptomless but infected banana planting material, movement of machinery with adhering infested soil, and the use of traditional banana-based packing material facilitate the spread of Foc.
A PCR-based detection tool for TR4 was used to analyze field samples from symptomatic and symptomless banana plants and soils collected in TR4-affected areas.
Foc TR4 was detected in all infected samples by conventional or nested PCR analyses.
The results will be used to develop and implement TR4 quarantine and management strategies.
Authors
M.A. Dita, C. Waalwijk, P. Mutua, A. Daly, P.F.L. Chang, B.M. Corcolon, L. Paiva, M. Souza, G.H.J. Kema
Keywords
management strategies, Panama disease, PCR analysis, quarantine pathogens
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