THE IMPACT OF GENOME AND 2,4-D ON CALLUS INDUCTION FROM IMMATURE MALE FLOWERS OF SEVEN BANANA CULTIVARS

To establish cell suspension of bananas, callus induction was initiated from immature male flowers.
Optimal concentration of 2,4-D on callus induction of seven banana cultivars was investigated.
Floral position 5-14 of young male flowers of Musa (ABB group) ‘Kluai Namwa’ (KNW), Musa (AA group) ‘Kluai Khai’ (KK), Musa (AAA group) ‘Kluai Hom Thong’ (KHT), Musa (AAA group) ‘Tumok’ (TM), Musa (BBB group) ‘Kluai Hin’ (KH), Musa (ABB group) ‘Kluai Hakmuk’ (KHM) and Musa (AA group) ‘Lep Mue Nang’ (LMN) were cultured on MS media supplemented with 0,2 or 4 mg L^-1 2,4-D incorporated with 1 mg L^-1 NAA, 1 mg L^-1 biotin and 30 g L^-1 sucrose.
Each position of flower was separately cultured on culture medium in the dark at 25°C. After 3 months, callus was obtained from flowers cultured on media plus 2 or 4 mg L^-1 2,4-D. Devoid of 2,4-D, callus could not be found in ‘KNW’, ‘KHM’ and ‘KK’, whereas ‘KH’, ‘KHT’, ‘TM’ and ‘LMN’ revealed callus formation.
Among the same genome, ‘KNW’ and ‘KHM’ responded to the culture media in the same way but ‘KK’ and ‘LMN’ verified different results.

International Symposium on Tropical and Subtropical Fruits

P. Karintanyakit, K. Suvittawat, W. Chinachit, B. Silayoi , P. Saratultad

surface sterilization, polyploidy, embryogenic culture

https://doi.org/10.17660/ActaHortic.2014.1024.33