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Articles

ASSESSING THE GENETIC VARIABILITY OF GRAPE CLONES

Article number

1046_48

Pages

357 – 361

Language

English

Abstract

In this work, to understand clonal genetic variability within six wine grape cultivars, we adopted three different methods.
Firstly, we carried out a Transposon Display analysis on 141 grape genotypes, which correspond to 47 clones (both registered and biotypes) belonging to the Pinot Noir, Pinot Gris, Pinot Blanc, Meunier, Teroldego and Gewürztraminer cultivars in 3 biological replicates (plants). We tested them using 17 primers targeting specific regions of 6 different Transposable Element families.
Secondly, by using 6 triallelic SSR markers we have analysed four tissues (leaf, berry skin, flesh and root) of the 19 registered clones.
Finally, we have genotyped the same set of samples by the SNPlex^TM Genotyping System, testing 430 electronic SNPs identified in coding and non-coding regions of the Pinot Noir ENTAV 115 grape genome.
Based on SNPlex results, we are currently resequencing the regions with a high level of polymorphism to confirm the presence of clonal mutations.
Here we report the results of the polymorphisms identified with the different approaches and putatively enabling molecular characterization of clones within international and local grape varieties.

Publication

X International Conference on Grapevine Breeding and Genetics

Authors

S. Vezzulli, L. Leonardelli, U. Malossini, M. Stefanini, R. Velasco, C. Moser

Keywords

Vitis vinifera, layer, mutation, Simple Sequence Repeat, Single Nucleotide Polymorphism, Transposon Display

Full text

https://doi.org/10.17660/ActaHortic.2014.1046.48

Online Articles (95)

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10. DEVELOPMENT OF MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE IN GRAPEVINES

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14. THE INTERACTION OF ELSINOË AMPELINA WITH VITIS VINIFERA

15. BERRY CRACKING CAUSED BY POWDERY MILDEW (UNCINULA NECATOR) INFECTION TO ‘FUJIMINORI’ GRAPEVINES (VITIS SP.)

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Daeil Kim | Sung-Il Oh | In-Chang Son

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18. ASSESSMENT OF THE BIOLOGICAL CHARACTERISTICS OF XYLELLA FASTIDIOSA IN XYLEM FLUID FROM A PIERCE’S DISEASE (PD) RESISTANT GRAPEVINE

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20. SCREENING GRAPE HYBRID FAMILIES WITH MOLECULAR MARKERS LINKED TO RESISTANCE GENES

21. THE GENETIC MAPPING OF XIPHINEMA INDEX RESISTANCE DERIVED FROM VITIS ARIZONICA

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22. CLONING AND FUNCTIONAL CHARACTERISATION OF A PUTATIVE POWDERY MILDEW SUSCEPTIBILITY GENE IN GRAPEVINE

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24. GENE SILENCING AS A STRATEGY TO INDUCE GRAPEVINE FANLEAF VIRUS (GFLV) RESISTANCE IN GRAPEVINE ROOTSTOCKS

25. PROGRESS IN GRAPEVINE BREEDING

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28. TETRAPLOID TABLE GRAPE BREEDING IN JAPAN

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29. DEVELOPMENT OF AUSTRALIAN ROOTSTOCKS WITH ROOT-KNOT NEMATODE RESISTANCE AND LOW POTASSIUM TRANSPORT

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73. ANTHOCYANIN PROFILING OF THE BERRY SKINS OF FIVE VITIS AMURENSIS GRAPES AND ONE RELATED HYBRID CULTIVAR

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74. CHANGES OF CONTENT AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS DURING GIBBERELLIN-INDUCED DEVELOPMENT OF SEEDLESS MUSCAT GRAPEVINES

Shu-fen Tian

75. MARKER-ASSISTED BREEDING: IDENTIFICATION OF MONOTERPENSYNTHASES IN GRAPEVINE (VITIS VINIFERA) AND THEIR POTENTIAL AS MARKERS IN BREEDING

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78. INTRAVARIETAL VARIABILITY OF ‘CRLJENAK KASTELANSKI’ AND ITS RELATIONSHIP WITH ‘ZINFANDEL’ AND ‘PRIMITIVO’ SELECTIONS

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81. PHENOTYPIC DIVERGENCE AMONG GRAPEVINE ACCESSIONS OF THE GERMPLASM COLLECTION AT IAC

82. BRAZILIAN GRAPE GERMPLASM BANK: PHENOLOGY AND INCIDENCE OF MAIN FUNGAL DISEASES

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83. GRAPEVINE VARIETY DETERMINATION FROM HERBARIUM AND ARCHEOLOGICAL SPECIMENS

N. Malenica | E. Maletic | S. Simon | I. Pejic

84. PLASTID DNA SEQUENCE DIVERSITY IN A WORLDWIDE SET OF GRAPEVINE CULTIVARS (VITIS VINIFERA L. SUBSP. VINIFERA)

85. ANALYSIS OF GRAPE ROOTSTOCKS BY MICROSATELLITE MARKERS

86. ANALYSIS OF PINOT CULTIVARS BY MICROSATELLITE MARKERS

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87. ITALIAN WILD GRAPEVINE: A STATE OF THE ART ON GERMPLASM AND CONSERVATION IN 2010; THE YEAR OF BIODIVERSITY

88. ‘RIBOLLA GIALLA’ FROM NORTH EASTERN ITALY, ‘REBULA’ FROM NORTHERN BALKANS AND ‘ROBOLA’ FROM IONIAN ISLANDS; DO THEY BELONG TO THE SAME POPULATION VARIETY OR ARE THEY GENETICALLY DIFFERENT?

89. PROTEOMIC ANALYSIS AMONG DIFFERENT AGLIANICO ECOTYPES

90. VITICULTURAL PERFORMANCES OF DIFFERENT ‘CABERNET SAUVIGNON’ CLONES

91. THE SOFTWARE FOR A ‘UNIVERSAL GRAPEVINE DATABASE’

C. D'Onofrio | G. Scalabrelli

92. MOLECULAR CHARACTERIZATION OF THE GRAPEVINE GERMPLASM COLLECTION HELD AT THE FONDAZIONE EDMUND MACH

93. ANALYSIS OF GENETIC STRUCTURE OF TWELVE SICILIAN GRAPEVINE CULTIVARS

94. AN AFLP-MARKER STUDY OF THE VITIS VINIFERA L. CULTIVAR ‘WHITE RIESLING’ COMPRISING 86 CLONES TO INVESTIGATE THE STABILITY OF CLONES

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95. SKIN PROTEOMIC COMPARISON AMONG FOUR GRAPE CULTIVARS WITH DIFFERENT ANTHOCYANIN CONTENTS