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Articles

RIPENING-RELATED ETHYLENE RESPONSIVE FACTOR CHARACTERIZATION BY AGROBACTERIUM-MEDIATED GENE TRANSFER IN GRAPEVINE

Article number

1046_68

Pages

499 – 503

Language

English

Abstract

Knowledge of the regulatory circuits and of the transcription factors involved in ripening in grape is lacking.
There is evidence of ethylene playing a role in fruit ripening and we have recently identified four ethylene responsive factor (ERF) genes which are transcriptionally modulated at the véraison phase.
Their gene expression profiles suggest an involvement in ripening regulation.
In order to understand the function of the VvERF1 gene in grape, its over-expression and silencing were carried out by gene transfer technology in Vitis vinifera Brachetto. The complete VvERF1 coding sequence was cloned in pK7WG2 vector for over-expression, whereas a 209 bp VvERF1 specific sequence was cloned in pK7GWIWG2 (II) for gene silencing.
We regenerated 20 plants putatively over-expressing VvERF1 and 5 plants putatively silencing VvERF1. According to molecular analysis, five of them contained the exogene VvERF1 gene under 35S promoter control.
Further characterization is in progress in the view of assessing the over expression of this exogene.

Publication

X International Conference on Grapevine Breeding and Genetics

Authors

A. Dal Ri, L. Dalla Costa, V. Poletti, L. Martinelli , C. Moser

Keywords

Vitis vinifera, berry ripening, ethylene responsive factors, embryo regeneration

Full text

https://doi.org/10.17660/ActaHortic.2014.1046.68

Online Articles (95)

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5. IDENTIFICATION OF AN R2R3 MYB TRANSCRIPTION FACTOR INVOLVED IN THE REGULATION OF THE STILBENE SYNTHASE PATHWAY IN GRAPEVINE

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7. SEQUENCING OF THE PHYLLOXERA RESISTANCE LOCUS RDV1 OF CULTIVAR ‘BÖRNER’

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8. DISSECTING THE GENETIC DETERMINANTS OF POWDERY MILDEW RESISTANCE IN GRAPE

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Wenping Qiu | W. Gassmann | Fei Gao | Kashmir Singh | Yiming Zhang

10. DEVELOPMENT OF MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE IN GRAPEVINES

11. SOURCES OF PENETRATION AND PCD-MEDIATED RESISTANCE TO GRAPEVINE POWDERY MILDEW IN THE VITACEAE FAMILY

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14. THE INTERACTION OF ELSINOË AMPELINA WITH VITIS VINIFERA

15. BERRY CRACKING CAUSED BY POWDERY MILDEW (UNCINULA NECATOR) INFECTION TO ‘FUJIMINORI’ GRAPEVINES (VITIS SP.)

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Daeil Kim | Sung-Il Oh | In-Chang Son

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18. ASSESSMENT OF THE BIOLOGICAL CHARACTERISTICS OF XYLELLA FASTIDIOSA IN XYLEM FLUID FROM A PIERCE’S DISEASE (PD) RESISTANT GRAPEVINE

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20. SCREENING GRAPE HYBRID FAMILIES WITH MOLECULAR MARKERS LINKED TO RESISTANCE GENES

21. THE GENETIC MAPPING OF XIPHINEMA INDEX RESISTANCE DERIVED FROM VITIS ARIZONICA

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22. CLONING AND FUNCTIONAL CHARACTERISATION OF A PUTATIVE POWDERY MILDEW SUSCEPTIBILITY GENE IN GRAPEVINE

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23. TOWARDS UNDERSTANDING THE MECHANISM OF HOST RESISTANCE TO DOWNY MILDEW DISEASE OF GRAPEVINE BY USING GENOME WIDE EXPRESSION PROFILING ANALYSIS

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24. GENE SILENCING AS A STRATEGY TO INDUCE GRAPEVINE FANLEAF VIRUS (GFLV) RESISTANCE IN GRAPEVINE ROOTSTOCKS

25. PROGRESS IN GRAPEVINE BREEDING

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28. TETRAPLOID TABLE GRAPE BREEDING IN JAPAN

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29. DEVELOPMENT OF AUSTRALIAN ROOTSTOCKS WITH ROOT-KNOT NEMATODE RESISTANCE AND LOW POTASSIUM TRANSPORT

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67. DRAWING LINKS FROM TRANSCRIPTOME TO METABOLITES: THE EVOLUTION OF MUSCAT AROMA IN THE RIPENING BERRY

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72. THE AROMA-FORMING SUBSTANCES OF HYBRID SEEDLINGS OF GRAPE

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73. ANTHOCYANIN PROFILING OF THE BERRY SKINS OF FIVE VITIS AMURENSIS GRAPES AND ONE RELATED HYBRID CULTIVAR

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74. CHANGES OF CONTENT AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS DURING GIBBERELLIN-INDUCED DEVELOPMENT OF SEEDLESS MUSCAT GRAPEVINES

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75. MARKER-ASSISTED BREEDING: IDENTIFICATION OF MONOTERPENSYNTHASES IN GRAPEVINE (VITIS VINIFERA) AND THEIR POTENTIAL AS MARKERS IN BREEDING

76. FUNCTIONAL CHARACTERIZATION OF TERPENE SYNTHASES OF ‘AROMATIC’ AND ‘NON-AROMATIC’ GRAPEVINE CULTIVARS

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77. GENEALOGY INVESTIGATION IN OVER 2,300 GRAPEVINE CULTIVARS (VITIS VINIFERA)

T. Lacombe | V. Laucou | M. Di Vecchi-Staraz | P. This | J.-M. Boursiquot

78. INTRAVARIETAL VARIABILITY OF ‘CRLJENAK KASTELANSKI’ AND ITS RELATIONSHIP WITH ‘ZINFANDEL’ AND ‘PRIMITIVO’ SELECTIONS

79. MOLECULAR SURVEY OF GEORGIAN TRADITIONAL GRAPEVINE GENETIC RESOURCES

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82. BRAZILIAN GRAPE GERMPLASM BANK: PHENOLOGY AND INCIDENCE OF MAIN FUNGAL DISEASES

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83. GRAPEVINE VARIETY DETERMINATION FROM HERBARIUM AND ARCHEOLOGICAL SPECIMENS

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84. PLASTID DNA SEQUENCE DIVERSITY IN A WORLDWIDE SET OF GRAPEVINE CULTIVARS (VITIS VINIFERA L. SUBSP. VINIFERA)

85. ANALYSIS OF GRAPE ROOTSTOCKS BY MICROSATELLITE MARKERS

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87. ITALIAN WILD GRAPEVINE: A STATE OF THE ART ON GERMPLASM AND CONSERVATION IN 2010; THE YEAR OF BIODIVERSITY

88. ‘RIBOLLA GIALLA’ FROM NORTH EASTERN ITALY, ‘REBULA’ FROM NORTHERN BALKANS AND ‘ROBOLA’ FROM IONIAN ISLANDS; DO THEY BELONG TO THE SAME POPULATION VARIETY OR ARE THEY GENETICALLY DIFFERENT?

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90. VITICULTURAL PERFORMANCES OF DIFFERENT ‘CABERNET SAUVIGNON’ CLONES

91. THE SOFTWARE FOR A ‘UNIVERSAL GRAPEVINE DATABASE’

C. D'Onofrio | G. Scalabrelli

92. MOLECULAR CHARACTERIZATION OF THE GRAPEVINE GERMPLASM COLLECTION HELD AT THE FONDAZIONE EDMUND MACH

93. ANALYSIS OF GENETIC STRUCTURE OF TWELVE SICILIAN GRAPEVINE CULTIVARS

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95. SKIN PROTEOMIC COMPARISON AMONG FOUR GRAPE CULTIVARS WITH DIFFERENT ANTHOCYANIN CONTENTS