Articles
REACTION OF SIX PRUNUS ROOTSTOCKS TO PLUM POX VIRUS IN PLOVDIV, BULGARIA
Article number
1063_15
Pages
111 – 116
Language
English
Abstract
The study aimed to score susceptibility of six Prunus rootstocks under high natural Plum pox virus (PPV) infection, in Plovdiv, Bulgaria.
The investigation included following rootstocks: Adesoto 101, Docera 6, Garnem, Greenpac and as controls Nemaguard and Mariana GF 8.1. Enzyme-linked immunosorbent assay (ELISA) based on the use of the universal monoclonal antibody 5B-IVIA were applied to detect Plum pox virus (PPV) in plants.
ELISA was performed in two variants: double antibody sandwich (DAS) and indirect double antibody sandwich (DASI). Additionally, some of PPV infected rootstocks were tested with monoclonal antibodies AL and 4DG for serotyping viral isolates.
During the period of the investigation, the experimental rootstocks were analyzed in three successive vegetative cycles and in two dormancies.
Each plant was tested as individual sample collecting at least 6-7 leaves or dormant buds per plant.
After three vegetative cycles, the relative infection rate in the experimental plot reached to 49.57%. The summarized data from five successive serological tests demonstrated that the most contaminated rootstocks were Nemaguard (73.05%) and Mariana GF 8.1 (70.03%). Adesoto 101 also showed high susceptibility to PPV, as in this rootstock 54.4% level of infection was recorded.
Moderate susceptibility appeared in Greenpac(39.8%) and Garnem (39%). PPV was detected in several plants of hypersensitive rootstock like Docera 6. Only PPV serotype M was identified among the analyzed viral isolates.
In summary, infection rate in the nursery plot increased from season to season at the rate of approximately 11%. The correlation between ELISA data from the winter and the springtime tests proved the possibility for early reliable diagnostics of PPV in dormancy.
The investigation included following rootstocks: Adesoto 101, Docera 6, Garnem, Greenpac and as controls Nemaguard and Mariana GF 8.1. Enzyme-linked immunosorbent assay (ELISA) based on the use of the universal monoclonal antibody 5B-IVIA were applied to detect Plum pox virus (PPV) in plants.
ELISA was performed in two variants: double antibody sandwich (DAS) and indirect double antibody sandwich (DASI). Additionally, some of PPV infected rootstocks were tested with monoclonal antibodies AL and 4DG for serotyping viral isolates.
During the period of the investigation, the experimental rootstocks were analyzed in three successive vegetative cycles and in two dormancies.
Each plant was tested as individual sample collecting at least 6-7 leaves or dormant buds per plant.
After three vegetative cycles, the relative infection rate in the experimental plot reached to 49.57%. The summarized data from five successive serological tests demonstrated that the most contaminated rootstocks were Nemaguard (73.05%) and Mariana GF 8.1 (70.03%). Adesoto 101 also showed high susceptibility to PPV, as in this rootstock 54.4% level of infection was recorded.
Moderate susceptibility appeared in Greenpac(39.8%) and Garnem (39%). PPV was detected in several plants of hypersensitive rootstock like Docera 6. Only PPV serotype M was identified among the analyzed viral isolates.
In summary, infection rate in the nursery plot increased from season to season at the rate of approximately 11%. The correlation between ELISA data from the winter and the springtime tests proved the possibility for early reliable diagnostics of PPV in dormancy.
Publication
Authors
S. Milusheva , V. Bozhkova
Keywords
stone fruit rootstocks, natural infection, Sharka disease, ELISA
Online Articles (25)