Articles
SSR MARKERS AS TOOLS FOR IDENTIFYING PPV RESISTANT APRICOT HYBRIDS IN SEGREGATING POPULATIONS
Article number
1063_19
Pages
137 – 141
Language
English
Abstract
Selection methods for PPV resistant apricot are mainly based on tracking the presence or absence of virus in the plant tissue.
The detection of the gene responsible for the PPV resistance seems to be much more reasonable.
It could be possible, if the gene of interest was known and appropriate markers for MAS selection were available.
Regarding the location of the PPV resistance gene a general consensus is formed.
According to that, the responsible gene is located in the Prunus reference genome map within the first linkage group at a distance of 20-40 cM. Several SSR markers which could be potentially useful are mapped in this region.
Based on the results of Zhebentyayeva et al. (2008) we have tested five SSR markers (Aprigms 18; Aprigms 24; ssrPaCITA5; ssrPaCITA17; EPDCU5100) on resistant and susceptible apricot cultivars.
The amplified PCR products were run in an automated sequencer ABI PRISM 3100 Genetic Analyzer and band scoring was analysed using Peak Scanner software.
Two (ssrPaCITA5; EPDCU5100) markers were found to be suitable for identification of PPV resistant cultivars.
We have also assaysed individuals from two hybrid populations obtained from the crossings of resistant and susceptible parents with ssrPaCITA5 and EPDCU5100 primers.
Populations derived from the crossings of Goldrich × M604, and Aurora × Ceglédi óriás. Goldrich and Aurora cultivars were used as the PPV resistance source.
The amplified PCR products were separated in high resolution polyacrylamide gel and visualized by silver staining.
The SSRs of sizes potentially linked with the PPV resistance were present in 50% of the individuals.
The detection of the gene responsible for the PPV resistance seems to be much more reasonable.
It could be possible, if the gene of interest was known and appropriate markers for MAS selection were available.
Regarding the location of the PPV resistance gene a general consensus is formed.
According to that, the responsible gene is located in the Prunus reference genome map within the first linkage group at a distance of 20-40 cM. Several SSR markers which could be potentially useful are mapped in this region.
Based on the results of Zhebentyayeva et al. (2008) we have tested five SSR markers (Aprigms 18; Aprigms 24; ssrPaCITA5; ssrPaCITA17; EPDCU5100) on resistant and susceptible apricot cultivars.
The amplified PCR products were run in an automated sequencer ABI PRISM 3100 Genetic Analyzer and band scoring was analysed using Peak Scanner software.
Two (ssrPaCITA5; EPDCU5100) markers were found to be suitable for identification of PPV resistant cultivars.
We have also assaysed individuals from two hybrid populations obtained from the crossings of resistant and susceptible parents with ssrPaCITA5 and EPDCU5100 primers.
Populations derived from the crossings of Goldrich × M604, and Aurora × Ceglédi óriás. Goldrich and Aurora cultivars were used as the PPV resistance source.
The amplified PCR products were separated in high resolution polyacrylamide gel and visualized by silver staining.
The SSRs of sizes potentially linked with the PPV resistance were present in 50% of the individuals.
Publication
Authors
D. Balázs, , Z. György, A. Gutermuth , A. Pedryc
Keywords
PPV, apricot, plant breeding, SSR markers
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