Articles
DEVELOPMENT OF PEACH PROTOPLAST CULTURE FOR PLUM POX VIRUS RESEARCH
Article number
1063_8
Pages
71 – 75
Language
English
Abstract
Research was conducted to isolate protoplasts and establish protoplast culture for peach and the system is aimed to be used for different studies of Plum pox virus research.
The results showed that viable protoplasts can be successfully isolated from both cotyledons and in vitro cultured leaves.
The viability of isolated protoplasts reached up to 90%, as analyzed by fluorescein diacetate staining.
The protoplast yield was different using leaves and cotyledons.
Also, optimal osmoticum treatment using mannitol in culture medium is important for protoplast isolation and yield.
Mannitol at 9% appeared to be optimal for higher protoplast yield with 7.8×105/ml.
The time required for enzymatic digestion of leaf and cotyledon explants differed and it took shorter time for enzyme digestion of leaves comparing to cotyledons.
The results showed that viable protoplasts can be successfully isolated from both cotyledons and in vitro cultured leaves.
The viability of isolated protoplasts reached up to 90%, as analyzed by fluorescein diacetate staining.
The protoplast yield was different using leaves and cotyledons.
Also, optimal osmoticum treatment using mannitol in culture medium is important for protoplast isolation and yield.
Mannitol at 9% appeared to be optimal for higher protoplast yield with 7.8×105/ml.
The time required for enzymatic digestion of leaf and cotyledon explants differed and it took shorter time for enzyme digestion of leaves comparing to cotyledons.
Publication
Authors
H. Fang, Q. Lu, A. Wang , L. Tian
Keywords
Prunus persica, tissue culture, virus
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