Articles
Dissipation rate of iprodione in sour cherry fruits
Article number
1139_126
Pages
739 – 744
Language
English
Abstract
The objectives of this work were the validation of a method for the analysis of iprodione residues in sour cherry fruits and estimation of dissipation rate in real fruit samples during seven days preharvest interval (PHI) in Serbia.
Samples were prepared using QuEChERS methodology and quantification was performed by high-performance liquid chromatography with diode array detector, with separation on a Zorbax Eclipse C18 column.
The mobile phase was acetonitrile/water (55:45, v/v) with a flow rate of 0.9 mL min-1 and detection wavelength of 250 nm.
The retention time of iprodione was 5.941 min.
Validation of the method was performed fulfilling the SANCO/12571/2013 criteria.
The QuEChERS method showed good recoveries (71.5-99.5%) at five concentration levels, from LOQ to 4.5 mg kg-1. Good results were obtained in terms of repeatability (RSDr=2.5%) and linearity (r2=0.998). The limit of detection (0.02 mg kg-1) and limit of quantification (0.06 mg kg-1) of the method were far below the maximum residual levels (MRLs) set by the EU and Serbian legislation for this fungicide in sour cherries (3 mg kg-1). Field trial was conducted according to standard OEPP method for experimental design and data analysis.
Iprodione was applied at the manufacturer’s recommended concentration.
Fruit samples were collected 1 h after the application and every two days until the expiration of the waiting period.
Iprodione dissipated rapidly, with the half-life (t1/2) of 3.30 days in cherry fruits.
Samples were prepared using QuEChERS methodology and quantification was performed by high-performance liquid chromatography with diode array detector, with separation on a Zorbax Eclipse C18 column.
The mobile phase was acetonitrile/water (55:45, v/v) with a flow rate of 0.9 mL min-1 and detection wavelength of 250 nm.
The retention time of iprodione was 5.941 min.
Validation of the method was performed fulfilling the SANCO/12571/2013 criteria.
The QuEChERS method showed good recoveries (71.5-99.5%) at five concentration levels, from LOQ to 4.5 mg kg-1. Good results were obtained in terms of repeatability (RSDr=2.5%) and linearity (r2=0.998). The limit of detection (0.02 mg kg-1) and limit of quantification (0.06 mg kg-1) of the method were far below the maximum residual levels (MRLs) set by the EU and Serbian legislation for this fungicide in sour cherries (3 mg kg-1). Field trial was conducted according to standard OEPP method for experimental design and data analysis.
Iprodione was applied at the manufacturer’s recommended concentration.
Fruit samples were collected 1 h after the application and every two days until the expiration of the waiting period.
Iprodione dissipated rapidly, with the half-life (t1/2) of 3.30 days in cherry fruits.
Publication
Authors
S. Lazić, D. Sunjka, S. Vuković, Z. Bjelica
Keywords
half-life, dicarboximide fungicide, Monilia laxa, Prunus cerasus, field trial
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