Home > VIII International Strawberry Symposium > Lessons learned from DNA-based tool development and use in a genebank

Articles

Lessons learned from DNA-based tool development and use in a genebank

Article number

1156_4

Pages

25 – 36

Language

English

Abstract

In 2002, a molecular genetics laboratory was established at the United States Department of Agriculture Agricultural Research Service (USDA-ARS), National Clonal Germplasm Repository (NCGR), in Corvallis, Oregon.
This facility houses the US national genebank for strawberry (Fragaria L.). A main objective of the molecular genetics laboratory is to characterize Fragaria genetic resources.
Plants from this collection have been analyzed using DNA-based tools for species identification, phylogenetic analyses, diversity assessment, identity and parentage confirmation, and development and validation of markers associated with traits of economic importance.
The development and application of microsatellite or simple sequence repeat (SSR) markers, single nucleotide polymorphism (SNP) markers, and DNA sequences in characterizing these strawberry genetic resources will be described.
We will also discuss the lessons learned from these studies and future plans for molecular characterization in Fragaria.

Publication

VIII International Strawberry Symposium

Authors

N.V. Bassil, K.E. Hummer, C.E. Finn

Keywords

genetic resources, identity verification, single nucleotide polymorphism markers, microsatellite markers

Full text

https://doi.org/10.17660/ActaHortic.2017.1156.4

Online Articles (140)

1. Advances in strawberry breeding at the University of Florida

2. The current progress in strawberry breeding in China

3. Genomics tools available for unravelling mechanisms underlying agronomical traits in strawberry with more to come

4. Lessons learned from DNA-based tool development and use in a genebank

N.V. Bassil | K.E. Hummer | C.E. Finn

5. The Fragaria species native to China and their geographical distribution

J.J. Lei | L. Xue | R.X. Guo | H.P. Dai

6. Breeding F₁ hybrid day-neutral strawberries in eastern North America

A. Dale | B.M. Santos | C.K. Chandler | B.R. Hughes | T. Taghavi

7. ‘Yotsuboshi’, a new F₁ hybrid strawberry of seed propagation type for year-round production

8. Domestication effects in European breeding history of strawberry demonstrated by aroma compound pattern

K. Olbricht | D. Ulrich

9. Progress in strawberry breeding at NIAB-EMR, East Malling, UK

10. Development and evaluation of the Axiom^® IStraw35 384HT array for the allo-octoploid cultivated strawberry Fragaria ×ananassa

11. Molecular approaches for monitoring clonal fidelity and epigenetic variation in in vitro-derived strawberry plants

S.C. Debnath

12. A high-throughput genotyping system combining rapid DNA extraction and high-resolution melting analysis in allo-octoploid strawberry

13. Functional analyses of cultivated strawberry FT and TFL1 homologs

14. Studies on diversity of wild Fragaria species in Yunnan, China

J. Wang | H.M. Yu | W.J. Cai | M.Z. Zhao

15. Seeking the northern boundary of the Cascade strawberry

K.E. Hummer | T.M. Davis

16. Morphological analysis of Fragaria chiloensis accessions and their relationship as parents of F. × ananassa hybrid

M. Gambardella | S. Sanchez | J. Grez

17. Current strawberry programmes at the Beijing Academy of Agriculture and Forestry Sciences

18. A new strawberry cultivar ‘Zijin Jiuhong’

M.Z. Zhao | Z.W. Wang | H.M. Yu | Q.L. Wang | W.J. Cai

19. Strawberry shape: phenotypic variation in length and width

A.R. Jamieson

20. Two new pink-flowered strawberry cultivars ‘Sijihong’ and ‘Xiaotaohong’

L. Xue | J.J. Lei | H.P. Dai

21. Cultivars developed in the strawberry breeding program of Fresas Nuevos Materiales S.A.

A. Refoyo | J.M. Arenas

22. Verification of linkage group using PRINS labeling technique in cultivated strawberry

K. Tantivit | S. Isobe | P. Nathewet | N. Okuda | T. Yanagi

23. Advances in strawberry breeding in Mexico: genotypes selection with high productivity, good fruit quality, and tolerance to the local viral complex and Fusarium oxysporum

P.A. Dávalos-González | A.E. Jofre-Garfias | L.F. Díaz-Espino | L.A. Mariscal-Amaro | M.G. López-Pérez

24. New strawberry cultivars and breeding activities in Brazil

25. Characterization of strawberry cultivars by SSR and CAPS markers

S. Günaydın | S. Kafkas

26. Updates on Italian strawberry breeding programs coordinated by CREA-FRF

27. ‘Malling Centenary’, a short-day strawberry cultivar from NIAB-EMR

28. A new strawberry cultivar ‘Shimei 8’

L. Yang | L. Li | L. Yang | J.-J. Zhang | H. Dong

29. Irrigation management based on soil matric potential improves water use efficiency of field-grown strawberries in California

30. Vigorous strawberry lines developed from recurrent selection under forcing culture

S. Kataoka | Y. Noguchi

31. Fragaria viridis Weston: diversity and breeding potential of an underutilised strawberry species

P. Gruner | D. Ulrich | C. Neinhuis | K. Olbricht

32. Fragaria germplasm: the “Professor Staudt Collection”

K. Olbricht

33. Fruit diversity in a natural European habitat of Fragaria moschata Weston

34. Electrical conductivity of the nutrient solution: implications for flowering and yield in day-neutral cultivars

N. Gallace | M. Boonen | P. Lieten | D. Bylemans

35. Strawberry cultivation on substrate: comparison of a continuous cultivation and a fresh spring planting

E. Braet | J. Simoen | J. Hanssens

36. The dynamic characteristics of soil enzyme activity and nematode diversity in replanted strawberry rhizosphere soil

W.H. Li | Q.Z. Liu

37. Vegetative and crop responses of strawberry to elevated hill cultivation systems in high latitude conditions

S.T. Karhu | P.K. Parikka

38. Six years of strawberry trials in commercial fields demonstrate that an extract of the brown seaweed Ascophyllum nodosum improves yield of strawberries

D. Holden | R. Ross

39. The effect of nitrogen on fruit shape and production in short day ‘Opera’ strawberry trayplants

P. Lieten | N. Gallace

40. Comparison of manual and automatic irrigation systems in strawberries

A. Ançay | V. Michel | C.A. Baroffio

41. Sawdust and bark-based substrates for tray plants strawberry production under greenhouse conditions

C. Depardieu | V. Prémont | C. Boily | J. Caron

42. Developing substrate culture strategies for the production of day-neutral strawberries

43. Analysis of nutrient contents and change rate of the drainage solution in hydroponics of strawberries

44. Effects of reducing fertilization and irrigation on strawberry productivity and fruit quality in Verona area

45. Effect of substrate and container type in the strawberry soilless cultivation

F. Massetani | G. Savini | D. Neri

46. Physiological performance reflecting in yield and quality of strawberry under vertical soilless culture system

B.N.S. Murthy | F. Karimi | R.H. Laxman

47. Tension-based irrigation management and leaching monitoring using electrical conductivity in strawberry production

48. Programming production potential and harvest spread through fertilisation in ‘Elsanta’ trayplants

P. Melis | K. Stoffels | M. Vervoort | T. Van Delm

49. Effects of fertigation on yield of day-neutral strawberry (Fragaria × ananassa) cultivars grown hydroponically in high tunnels

C. Sarraf | L. Gauthier | A. Gosselin | Y. Desjardins

50. Effects of runner removal on productivity and plant growth of two day-neutral strawberry cultivars in Ontario, Canada

B.R. Hughes | J. Zandstra | T. Taghavi | A. Dale

51. Effect of combined fertilization of NPK on the first inflorescence yield of ‘Ningfeng’ strawberry

52. Side-dressing fertilization on strawberry (Fragaria × ananassa) ‘Aromas’ at three locations in Huaura Valley – Peru

53. Effects of various calcium nitrate application on ester profiles of some strawberry cultivars

M.A. Sarıdaş | S.P. Kargi | M. Zarifikhosroshahi | E. Kurt | E. Kafkas

54. The effects of strawberry bioactive compounds on human health

55. A10-48-3 and A7-32-10, two strawberry selections with well-balanced nutritional and organoleptic quality

A.G. Pérez | R. García-Rodríguez | C. Sanz | A. Refoyo

56. Evaluation of vitamin C content in fruit and leaves of different strawberry genotypes

57. Evaluation of strawberry (Fragaria × ananassa Duch.) Alba sensorial and nutritional quality, and its in vitro effects against human breast cancer cells viability

58. Effects of in vitro gastrointestinal digestion on strawberry polyphenols stability

59. The photoprotective effects of strawberry-based cosmetic formulations on human dermal fibroblasts

S. Afrin | T.Y. Forbes-Hernandez | F. Giampieri | M. Battino | M. Gasparrini

60. Effects of three genetically-modified strawberry selections on human dermal fibroblasts exposed to AAPH-induced oxidative stress

61. The effect of an enzymatic digestion process on strawberry antioxidant capacity

T.Y. Forbes-Hernandez | M. Gasparrini | S. Afrin | M. Battino | F. Giampieri

62. Salt stress induces differential responses in yield, fruit quality, vegetative growth and physiological parameters in commercial (Fragaria × ananassa) and Chilean strawberry (F. chiloensis) genotypes

M. Garriga | P.D.S. Caligari | J.B. Retamales

63. Effect of long days and high temperatures in the nursery on the fruit production pattern of the short-day strawberry cultivar ‘Gariguette’

J.P. Bosc | F. Massetani

64. Induction and floral differentiation in white strawberry (Fragaria chiloensis subsp. chiloensis f. chiloensis)

J. Grez | F. Massetani | D. Neri | M. Gambardella

65. Investigations on the chemical composition of cuticular waxes in twelve Fragaria L. genotypes

H. Wagner | M. Riedel | M. Riederer | K. Olbricht

66. Physiology of strawberry plants under controlled environment: diurnal change in leaf net photosynthetic rate

K. Garcia | C. Kubota

67. Heat before flowering decreases albinism in strawberries

A. Dale | S. Pirgozliev | S. Kermasha

68. Selective inflorescence thinning as a tool to improve yield quality of everbearing strawberry

P. Zucchi | P. Martinatti | S. Conci | T. Pantezzi

69. Forecasting strawberry yields (‘eascape’) three weeks in advance: validation and optimisation of a method developed under commercial production conditions in Quebec

P. Deschênes | C. Boivin

70. Photoperiod and temperature interaction on inflorescence architecture and receptacle size of everbearing ‘Capri’ strawberry

P. Zucchi | P. Martinatti | T. Pantezzi | L. Giongo

71. Flowering responses of North American strawberry cultivars

K. Garcia | C. Kubota

72. Relationship between sugar content and °Brix as influenced by cultivar and ripening stages of strawberry

F. Pistón | A.G. Pérez | C. Sanz | A. Refoyo

73. Effects of GA₃ on vegetative growth in strawberry

74. Does nitrate accumulate in strawberry leaves as nutrient nitrate concentration increases?

F. Shafiee | T. Taghavi

75. Effect of chilling duration on runner production and vegetative growth of ‘Pajaro’ strawberry

T. Taghavi | M. Aghajani

76. Effect of fruit gas exchange on photosynthate accumulation and fruit growth of strawberries

77. Overcoming climatic limitations: cultivation systems and winter production under assimilation lighting resulting in year-round strawberries

T. Van Delm | K. Stoffels | P. Melis | M. Vervoort | R. Vanderbruggen

78. Controlled environment strategies for tipburn management in greenhouse strawberry production

M. Kroggel | C. Kubota

79. Covered soilless strawberry production in the field by raised substrate beds

M. Boonen | N. Gallace | D. Bylemans

80. Remote sensing as a useful tool in strawberry cultivation

81. Strawberry cultivar performance in high tunnels under sustainable and organic production practices in three climatic regions of Arkansas

M.E. Garcia | T. Ernst | D.T. Johnson | D.A. Dickey

82. Use of low tunnels to improve the productivity of day-neutral strawberry plants under the Québec climatic conditions

A. Van Sterthem | Y. Desjardins | L. Gauthier | Y. Medina | A. Gosselin

83. Preliminary results on the polyphenol content of strawberry (Fragaria × ananassa Duch. ‘Florida Fortuna’) fruits during ripening

E. Kafkas | S. Kafkas

84. Strawberry cultivation in Chinese solar greenhouse with three thermal walls in northern China

H.P. Dai | L. Xue | C.H. Tan | J.J. Lei

85. Influence of low tunnels on strawberry production time and yield

V. Laugale | S. Dane | L. Lepse | S. Strautina | I. Kalnina

86. Effects of organic substrate reuse on growth and yield of everbearing ‘Capri’strawberry

P. Zucchi | C.M.O. Longa | D. Bertoldi | P. Martinatti | T. Pantezzi

87. Assessment tool to compare the environmental, economic and social sustainability of strawberry production systems in Quebec

M. Gendron | V. Gravel | O. Carisse

88. Weed and pathogen control with steam in California strawberry production

89. Delizzimo^®: development of a sustainable strawberry production system in winter season

G.C.M. Bentvelsen | D. Souillat

90. Accomplishments and impacts of the National Strawberry Sustainability Initiative, 2013-2015

91. Yield and fruit quality of strawberry cultivars grown in organic farming in the mid-Adriatic area

F. Capocasa | F. Balducci | C. Martellini | A. Albanesi

92. Spreading of diseases in strawberry – legume intercropping

S. Dane | V. Laugale | D. ¿terne | G. Bim¿teine

93. ‘Renaissance’ of flavor: portrait of a new European strawberry cultivar

K. Olbricht | U. Gerischer | K. Weiß | D. Ulrich

94. Extending shelf life of strawberry using some pre-storage treatments

S. Samadi | A. Ghasemnezhad | J. Imani

95. Searching for chemical and sensory parameters for flavor enhancement in strawberry breeding

D. Ulrich | K. Olbricht

96. Correlation between phosphorus content and fruit quality in strawberries

Zhihong Zhang | Fei Cao | He Li | Xiaoming Li | Hongyan Dai

97. Effect of ethylene on postharvest strawberry fruit tissue biochemistry

F. Elmi | I. Pradas | R. Tosetti | K. Cools | L.A. Terry

98. Analysis of aroma compounds in new strawberry advanced genotypes

G. Bianchi | P. Lucchi | M.L. Maltoni | A.F. Fagherazzi | G. Baruzzi

99. Fragaria cascadensis K.E. Hummer: first investigation of volatile organic compounds of fruit

D. Ulrich | K. Olbricht | K. Weiß | K.E. Hummer

100. Identification and quantification of some fruit quality characteristics of new strawberry cultivars in Turkey

101. Identification of strawberry (Fragaria × ananassa ‘Rubygem’) volatiles using various SPME fibres by GC/MS

E. Kafkas | S. Kafkas

102. Rapid volatile metabolomics and genomics in large strawberry populations segregating for aroma

C.R. Barbey | K.M. Folta | V.M. Whitaker | S. Verma | J. Bai

103. Aroma profiles of organically grown ‘Benicia’ and ‘Albion’ strawberries

104. Integrated approach of powdery mildew control on strawberry cultivar ‘Elsanta’ in Belgium

P. Melis | K. Stoffels | M. Vervoort | T. Van Delm

105. Antifungal effect of Lamiaceae herbs on Fusarium wilt in strawberry

Y. Matsubara | J. Liu | S.I. Haque

106. Epidemiology of Erwinia pyrifoliae, a new pathogen on strawberry in The Netherlands

M. Wenneker | M. Bergsma-Vlami | J.J.M. van der Steen

107. DNA barcode identification and virulence testing of Phytophthora spp. and Pythium spp. isolated from soil of strawberry fields

A.R. Toljamo | L.J. Granlund | H.I. Kokko

108. Protecting fungicide chemistry used in Australian strawberry production for more sustainable control of powdery mildew and leaf blotch

109. Testing strawberry genotypes for resistance to Colletotrichum gloeosporioides in Queensland

110. Possibilities for biological control of red core (Phytophthora fragariae)

P.K. Parikka | M. Vestberg | S.T. Karhu | T. Haikonen | J. Hautsalo

111. Evaluation of a new biopesticide against angular leaf spot in a commercial operation system

C. Dubois | G. Arsenault-Labrecque | J. Pickford

112. Strawberry germplasm response to Fusarium oxysporum f. sp. fragariae under greenhouse conditions, and identification of sources for resistance in Mexico

A.L.A. Mariscal | P.A. Dávalos-Gonzáles | A.E. Jofre-Garfias | M.D. Ávila

113. Integrated control of strawberry powdery mildew

A.M. Hall | X. Jin

114. Strawberry cultivar and breeding lines susceptibility to Phytophthora crown and root rot in Huelva (Spain)

C. Borrero | A. Refoyo | C. Sanz | M. Avilés

115. Crown rot in strawberry caused by Pestalotiopsis

W. Van Hemelrijck | A. Ceustermans | J. Van Campenhout | P. Lieten | D. Bylemans

116. Isolation and expression profiling of FaCERK1 during Colletotrichum gloeosporioides infection of strawberry plants

117. The occurrence and control to strawberry anthracnose in China

118. Advances in characterization and epidemiology of strawberry viruses and phytoplasmas in Argentina

119. Biological control of root pathogens in strawberry cultivation in tunnel

S. Khalil | B. Svensson

120. A hybrid state space modelling study on effects of cultivation management on biological pest control for strawberry plants

I. Zamani | M. Hosseini Farahi

121. Silicon location in strawberry plants to improve resistance to powdery mildew (Podosphaera aphanis)

Y.P. Sun | A.M. Hall | Y.J. Huang | B. Liu

122. Current spotted wing drosophila IPM tactics and their practical implementation in berry crops in Switzerland

M. Dorsaz | F. Kuonen | C.A. Baroffio

123. Thrips control with predatory mites A. limonicus and A. swirskii in different strawberry cultivation systems

124. Toxicity of erythritol, a sugar alcohol and food additive, to Drosophila suzukii (Matsumara)

125. Pupation depth of spotted wing drosophila (Drosophila suzukii) and effects of field sanitation in Florida strawberries

J.M. Renkema | S. Devkota

126. Damage and potential of fungus gnat as a Fusarium vector in the strawberry plants in Korea

127. Matching commercial thrips predating phytoseids with the highly diversified climatic conditions of different strawberry production systems

128. Development and performance of soil-less systems for strawberry transplant production in Australia

M. Milinkovic | S.W. Mattner | L.T. House | F.C. Greenhalgh

129. Genetic stability of ‘Festival’ and ‘Rubigem’ cultivars in different subcultures by SSR markers

D. Ayvaz-Sönmez | S. Kafkas

130. Effects of NaCl and NaHCO₃ stress on the growth of in vitro culture seedlings of Fragaria × ananassa Duch.

X. Zhao | G. Li | L.J. Li | P.P. Hu | H.C. Zhou

131. Delizz^®: development of a strawberry product concept for the consumer gift market

G.C.M. Bentvelsen | D. Souillat

132. Productivity of strawberry plants growing under dry and wet conditions in subtropical Queensland

C.M. Menzel | L.A. Smith | J.A. Moisander

133. Strawberry growing in Turkey: current status and future prospects

E. Kafkas

134. Strawberry cultivation in southern Punjab Pakistan

135. Challenges and opportunities for early strawberry production in Greece: incorporating new cultivars in the production system

E. Tsormpatsidis | E. Vysini | T. Papanikolopoulos

136. Strategies to increase strawberry competitiveness among fruit growers, marketers and consumers in Kenya

M. Mwangi | A. Egesa | F. Matheri

137. Smallholder farmers’ logic to promote strawberry value chain in the rural Commune of Tsiafahy, Analamanga Régions, Antananarivo

138. Strawberry production progress in Brazil

139. Growth and production patterns of strawberries grown in the Atlantic coast of Argentina

D.S. Kirschbaum | E.G.A. Adlercreutz | N. Gariglio

140. Strawberry in South America: from the Caribbean to Patagonia