Establishing a protocol for dedifferentiation and redifferentiation of Tanacetum cinerariifolium leaves

Calli were induced from leaf fragments of pyrethrum plants grown from seeds of Japan origin on Murashige-Skoog (MS) medium supplemented with auxins (1‑naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)) and a cytokinin (6‑benzylaminopurine (BAP)). The hormone concentrations were optimized to yield green and healthy calli.
Green spots on such calli were transferred to auxin-free and hormone-free medium successively to redifferentiate leaves.
Pyrethrin contents were markedly reduced to below detectable levels by dedifferentiation.

II International Symposium on Pyrethrum

K. Sakamori, K. Matsuda

pyrethrins, callus, Tanacetum cineraiifolium, wound-induced volatiles

https://doi.org/10.17660/ActaHortic.2017.1169.22