Articles
In vitro shoots and micro-corms formation through indirect organogenesis of Moroccan saffron (Crocus sativus L.)
Article number
1184_14
Pages
97 – 108
Language
English
Abstract
Saffron (Crocus sativus L.) is an important plant because of its medicinal and aromatic characteristics.
Tissue culture of this specie, through direct or indirect organogenesis, can improve the quality and quantity of the saffron product, by the large production of healthy selected saffron corms.
The objective of this work is the determination of the best combination of plant growth regulators for callus production from three kinds of explant (upper parts of corm tissue, lower parts of carom tissue and segment of corm tissue without bud) and for plantlet regeneration from callus.
Callus was produced in Murashige and Skoog (MS) media with 3% (w/v) sucrose, 100 mg L-1 ascorbic acid and supplemented with different combinations of PGRs.
Maximum callus initiation was observed in the combination of 1 mg L-1 NAA and 1 mg L-1 BAP in the upper part of corms tissue.
The best combinations for shoot initiation were 2 mg L-1 TDZ with 0.5 mg L-1 NAA. For shoots number, the best treatment was 2.75 mg L-1 TDZ with 0.5 mg L-1 NAA. The incubation of explants in MS media under obscurity was more beneficial for corms and roots formation, even in the absence of plant growth regulators.
Tissue culture of this specie, through direct or indirect organogenesis, can improve the quality and quantity of the saffron product, by the large production of healthy selected saffron corms.
The objective of this work is the determination of the best combination of plant growth regulators for callus production from three kinds of explant (upper parts of corm tissue, lower parts of carom tissue and segment of corm tissue without bud) and for plantlet regeneration from callus.
Callus was produced in Murashige and Skoog (MS) media with 3% (w/v) sucrose, 100 mg L-1 ascorbic acid and supplemented with different combinations of PGRs.
Maximum callus initiation was observed in the combination of 1 mg L-1 NAA and 1 mg L-1 BAP in the upper part of corms tissue.
The best combinations for shoot initiation were 2 mg L-1 TDZ with 0.5 mg L-1 NAA. For shoots number, the best treatment was 2.75 mg L-1 TDZ with 0.5 mg L-1 NAA. The incubation of explants in MS media under obscurity was more beneficial for corms and roots formation, even in the absence of plant growth regulators.
Authors
K. Lagram, M. Ben El Caid, L.H. Atyane, L. Salaka, R. El Boullani, A. El Mousadik, M.A. Serghini
Keywords
saffron, corms, in vitro, organogenesis, Crocus sativus
Groups involved
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