Articles
Callus induction via thin cell layer culture of two native blackberry genotypes of Iran
Article number
1190_22
Pages
129 – 134
Language
English
Abstract
In this research, callogenesis was investigated from stem traverse thin cell layer (tTCL) segments of two native blackberry genotypes of Iran named ‘high thorny’ and ‘low thorny’. For callus initiation, Murashige and Skoog (MS) medium with different types and concentrations of plant growth regulators (PGRs) were used.
The results showed, in ‘high thorny’ genotype, the highest callus rate was achieved in MS medium supplemented with naphthaleneacetic acid (NAA), kinetin (KIN) and benzyladenine (BA) (each 1 mg L‐1). For improving the callus quality, two culture media, MS and Anderson (AN) supplemented with 0.2 mg L‐1 2,4-dichlorophenoxyacetic acid (2,4-D) or 0.5 mg L‐1 NAA were used in combination with different types of cytokinins.
Generally, the best callus quality (freshness, friability) was observed in ‘low thorny’ genotype on MS medium.
In ‘high thorny’ genotype; 2,4-D and NAA had the same effect on callus quality whilst in ‘low thorny’ genotype NAA was more effective than 2,4-D.
The results showed, in ‘high thorny’ genotype, the highest callus rate was achieved in MS medium supplemented with naphthaleneacetic acid (NAA), kinetin (KIN) and benzyladenine (BA) (each 1 mg L‐1). For improving the callus quality, two culture media, MS and Anderson (AN) supplemented with 0.2 mg L‐1 2,4-dichlorophenoxyacetic acid (2,4-D) or 0.5 mg L‐1 NAA were used in combination with different types of cytokinins.
Generally, the best callus quality (freshness, friability) was observed in ‘low thorny’ genotype on MS medium.
In ‘high thorny’ genotype; 2,4-D and NAA had the same effect on callus quality whilst in ‘low thorny’ genotype NAA was more effective than 2,4-D.
Authors
N. Sabooni, A. Shekafandeh
Keywords
callogenesis, PGRs, Rubus, tissue culture, tTCL
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