Application of homozygosis dominant SSR marker in longan hybrid authenticity identification

In this study, two F₁ longan hybrid populations named FDD265 and FDD97 were created in 2012, using ‘Daguocaopu’ as pollen parent, meanwhile ‘FD265’ and ‘FD97’ selected from ‘Fengliduo’ × ‘Dawuyuan’ (FD) F₁ population as maternal parents, respectively.
In order to obtain reliable hybrids, we developed to identify the hybrids using paternal homozygosis dominant marker.
Firstly, using the parental genomic DNA of FDD265 and FDD97 as templates, 88 and 90 of 274 pairs of SSR primers could amplify the paternal specific markers separately.
Secondly, these paternal specific markers were used to screen the inseparable loci using the self-crossed progenies of pollen parent ‘Daguocaopu’. Finally, 3 and 1 paternal homozygous dominant markers were selected for identifying 67 and 66 individuals of FDD265 and FDD97 populations, respectively.
The result showed that all hybrids of the two populations were identified as real hybrids with the high rates up to 100% hybridity.
This result indicates the accuracy and effectiveness of this method for identifying young hybrids using paternal homozygosis dominant marker.

V International Symposium on Lychee, Longan and Other Sapindaceae Fruits

Lili Jia, Chen Dan-lin, Liu Chao-ji, P. Li-jia, Wang Ya-ting, Hu Lei, Wu Zhenxian, Hu Gui-bing, Fu Jia-xin, Liu Cheng-ming

longan, SSR, hybrid identification, male specific homozygosis dominant marker

https://doi.org/10.17660/ActaHortic.2018.1211.30