Articles
Somatic embryogenesis from oil palm zygotic embryos: the impact of medium components and culture conditions
Article number
1224_4
Pages
21 – 30
Language
English
Abstract
The impact of various medium components and culture conditions on somatic embryogenesis in oil palm was assessed.
Immature zygotic embryos were cultured on a modified Murashige and Skoog (MS) medium supplemented with 2 g L-1 activated charcoal.
Factors that were investigated include the auxin type and concentration, the carbon source and concentration, the gelling agent concentration and the culture medium pH. Especially the concentration and type of carbon source seemed to have a large influence on callus formation, while changing the concentration of auxins only caused minor differences in reaction.
Glucose monohydrate was preferred over sucrose.
High concentrations of carbon inhibited callus formation.
Reducing medium strength by lowering gelling agent concentration only caused a slight increase in callus formation, while lowering culture medium pH did not influence callus formation.
However, a low pH during the callus induction phase might stimulate the formation of somatic embryos.
Gained knowledge about tissue culture of zygotic embryos can hopefully be applied to other explants (e.g., immature leaves) that are used for commercial micropropagation of oil palm.
Immature zygotic embryos were cultured on a modified Murashige and Skoog (MS) medium supplemented with 2 g L-1 activated charcoal.
Factors that were investigated include the auxin type and concentration, the carbon source and concentration, the gelling agent concentration and the culture medium pH. Especially the concentration and type of carbon source seemed to have a large influence on callus formation, while changing the concentration of auxins only caused minor differences in reaction.
Glucose monohydrate was preferred over sucrose.
High concentrations of carbon inhibited callus formation.
Reducing medium strength by lowering gelling agent concentration only caused a slight increase in callus formation, while lowering culture medium pH did not influence callus formation.
However, a low pH during the callus induction phase might stimulate the formation of somatic embryos.
Gained knowledge about tissue culture of zygotic embryos can hopefully be applied to other explants (e.g., immature leaves) that are used for commercial micropropagation of oil palm.
Authors
S. Weckx, L. Maene
Keywords
Elaeis guineensis, tissue culture, callus induction, carbon source, pH, somatic embryogenesis
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