Articles
Antagonistic activity of bacterial rhizosphere from rootstocks of tomato and eggplant against Ralstonia solanacearum
Article number
1270_39
Pages
321 – 326
Language
English
Abstract
Tomato bacterial wilt disease caused by Ralstonia solanacearum could be controlled by grafting on resistant rootstocks.
However, antagonistic activity of rootstock rhizospheric bacteria against R. solanacearum has never been investigated.
Two local cultivars of tomato were grafted on rootstocks H-7996 and Eg-203. At 14 and 28 days after transplanting, population of fluorescent pseudomonads and Bacillus spp. in the rhizosphere of the grafted plants were investigated.
Fluorescent pseudomonads were plated onto King’s B medium and Bacillus spp. onto Yeast Peptone Glucose Agar medium.
Cycloheximide was added into each medium (100 ppm) to suppress fungal growth.
The isolated bacteria were tested for their antagonistic activity against R. solanacearum in vitro.
The population of fluorescent pseudomonads and Bacillus spp. was not significantly different between grafted and non-grafted plants.
The population of those bacteria increased tenfold from 14 to 28 days after transplanting.
The growth of R. solanacearum in vitro was suppressed by fluorescent pseudomonads and Bacillus spp.
The growth suppression was supposed to be bacteriostatic activity.
Isolated fluorescent pseudomonads suppressed growth of Bacillus spp.
On the contrary, isolated Bacillus spp. did not suppress the growth of fluorescent pseudomonads.
It is assumed that fluorescent pseudomonads and Bacillus spp. in the rhizosphere of grafted tomato plants may have played a role in bacterial wilt suppression.
However, antagonistic activity of rootstock rhizospheric bacteria against R. solanacearum has never been investigated.
Two local cultivars of tomato were grafted on rootstocks H-7996 and Eg-203. At 14 and 28 days after transplanting, population of fluorescent pseudomonads and Bacillus spp. in the rhizosphere of the grafted plants were investigated.
Fluorescent pseudomonads were plated onto King’s B medium and Bacillus spp. onto Yeast Peptone Glucose Agar medium.
Cycloheximide was added into each medium (100 ppm) to suppress fungal growth.
The isolated bacteria were tested for their antagonistic activity against R. solanacearum in vitro.
The population of fluorescent pseudomonads and Bacillus spp. was not significantly different between grafted and non-grafted plants.
The population of those bacteria increased tenfold from 14 to 28 days after transplanting.
The growth of R. solanacearum in vitro was suppressed by fluorescent pseudomonads and Bacillus spp.
The growth suppression was supposed to be bacteriostatic activity.
Isolated fluorescent pseudomonads suppressed growth of Bacillus spp.
On the contrary, isolated Bacillus spp. did not suppress the growth of fluorescent pseudomonads.
It is assumed that fluorescent pseudomonads and Bacillus spp. in the rhizosphere of grafted tomato plants may have played a role in bacterial wilt suppression.
Authors
T. Arwiyanto, S.D. Nurcahyanti, D. Indradewa, J. Widada
Keywords
tomato, rootstock, rhizosphere bacteria, grafting, Ralstonia solanacearum
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