Articles
Comparison of SRAP markers and pedigree data in assessing genetic relatedness among chrysanthemum cultivars
Article number
1283_11
Pages
71 – 82
Language
English
Abstract
Complete pedigree data are of critical importance for efficient conservation and utilization of genetic resources.
However, the pedigrees of many chrysanthemum cultivars are completely unknown, often reducing the breeding efficiency.
This study was aimed at comparing the effectiveness of sequence-related amplified polymorphism (SRAP) markers and pedigree data in measuring genetic relatedness among a working set of chrysanthemum cultivars.
A total of 121 polymorphic fragments were yielded by 17 SRAP primer combinations, with polymorphism information content (PIC) values ranging from 0.13 to 0.50 (mean 0.34) individually.
The SRAP-based genetic similarity varied from 0.38 to 0.95, and averaged at 0.61, indicative of a moderate low variability harbored in the investigated chrysanthemums.
The SRAP-based clustering grouped the investigated chrysanthemums well concordant to both flower type and ancestors, whereas the pedigree-based clustering showed congruence only to their ancestors.
In addition, the SRAP-based genetic similarities were generally consistent with a normal distribution, while the pedigree-based coefficients of co-ancestry displayed a biased distribution with most calculated at 0. Statistical analysis, however, showed a highly significant correlation (r=0.77, P<0.001) and a moderate significant linear relationship (R2=0.59) between the two measurements.
The findings highlight the potential of pedigree data in measuring genetic relatedness among chrysanthemums, despite being less informative than molecular markers.
However, the pedigrees of many chrysanthemum cultivars are completely unknown, often reducing the breeding efficiency.
This study was aimed at comparing the effectiveness of sequence-related amplified polymorphism (SRAP) markers and pedigree data in measuring genetic relatedness among a working set of chrysanthemum cultivars.
A total of 121 polymorphic fragments were yielded by 17 SRAP primer combinations, with polymorphism information content (PIC) values ranging from 0.13 to 0.50 (mean 0.34) individually.
The SRAP-based genetic similarity varied from 0.38 to 0.95, and averaged at 0.61, indicative of a moderate low variability harbored in the investigated chrysanthemums.
The SRAP-based clustering grouped the investigated chrysanthemums well concordant to both flower type and ancestors, whereas the pedigree-based clustering showed congruence only to their ancestors.
In addition, the SRAP-based genetic similarities were generally consistent with a normal distribution, while the pedigree-based coefficients of co-ancestry displayed a biased distribution with most calculated at 0. Statistical analysis, however, showed a highly significant correlation (r=0.77, P<0.001) and a moderate significant linear relationship (R2=0.59) between the two measurements.
The findings highlight the potential of pedigree data in measuring genetic relatedness among chrysanthemums, despite being less informative than molecular markers.
Authors
X.C. Yang, X.D. Yang, Y.Y. Wu, J.S. Su, H.B. Wang, Z.Y. Guan, F.D. Chen, W.M. Fang, F. Zhang
Keywords
chrysanthemum, genetic variability, pedigree, sequence-related amplified polymorphism
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