Articles
Association studies in roses reveal robust markers for flower traits
Article number
1283_27
Pages
199 – 208
Language
English
Abstract
Floral traits are the most important characteristics that determine the ornamental value of cultivated roses.
A number of studies have been conducted on qualitative and quantitative factors influencing floral traits but almost all of these studies were based on biparental populations.
Here we present data on markers generated through an association study in a set of 96 diverse rose genotypes for flower petal number and the verification of a marker in a set of independent populations.
For marker analysis, we used a recently designed Axiom SNP chip comprising 68,893 SNPs with additionally 281 SSRs, 400 AFLPs and 246 markers derived from candidate genes.
The mapping of markers significantly associated with petal number revealed clusters of associated markers indicating genomic regions associated with the trait on chromosome 1, 3, 5 and 6. One of these genomic regions on chromosome 3 is located in vicinity of the DOUBLE FLOWER locus, where a dominant gene controls simple versus double flower phenotypes (Hibrand Saint-Oyant et al., 2018). Genetic markers were developed, which can be considered as beneficial for marker-assisted selection in commercial breeding programmes in the future.
These markers for petal number are also starting points for functional genomic studies to identify the causal factors for the observed phenotypes.
A number of studies have been conducted on qualitative and quantitative factors influencing floral traits but almost all of these studies were based on biparental populations.
Here we present data on markers generated through an association study in a set of 96 diverse rose genotypes for flower petal number and the verification of a marker in a set of independent populations.
For marker analysis, we used a recently designed Axiom SNP chip comprising 68,893 SNPs with additionally 281 SSRs, 400 AFLPs and 246 markers derived from candidate genes.
The mapping of markers significantly associated with petal number revealed clusters of associated markers indicating genomic regions associated with the trait on chromosome 1, 3, 5 and 6. One of these genomic regions on chromosome 3 is located in vicinity of the DOUBLE FLOWER locus, where a dominant gene controls simple versus double flower phenotypes (Hibrand Saint-Oyant et al., 2018). Genetic markers were developed, which can be considered as beneficial for marker-assisted selection in commercial breeding programmes in the future.
These markers for petal number are also starting points for functional genomic studies to identify the causal factors for the observed phenotypes.
Authors
D.F. Schulz, M. Linde, T. Debener
Keywords
GWAS, SNP, KASP, MADS-box genes, marker-assisted breeding, petal number
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