Articles
Application of isoenzymes in selection of low vigour cherry genotypes
Article number
1289_18
Pages
125 – 130
Language
English
Abstract
This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes.
The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study.
For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction.
Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes.
The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards.
The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study.
For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction.
Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes.
The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards.
Publication
Authors
S. Čolić, I. Bakić, V. Rakonjac, D. Nikolić, M. Fotirić Akšić
Keywords
Prunus spp., cherry, low vigour, isoenzymes, electrophoresis, cluster analysis
Groups involved
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