Articles
Effect of a novel ABA receptor antagonist 3-hexylsulfanyl-ABA (AS6) on ascorbate metabolism in postharvest broccoli
Article number
1336_33
Pages
249 – 256
Language
English
Abstract
Broccoli is a perishable vegetable, which senesces rapidly after harvest.
During senescence, the florets of broccoli turn yellow, and the contents of the bioactive compounds decrease to low levels.
The occurrence of senescence significantly reduces the nutritional and commercial values of broccoli.
In this study, to delay the senescence of broccoli, we treated the broccoli with a novel ABA receptor antagonist 3-hexylsulfanyl-ABA (AS6) after harvest at room temperature, and its effects on broccoli senescence were investigated.
The results showed that the yellowing process was delayed by the treatment of AS6. On the 4th day after harvest, the florets displayed obvious yellowing in the control, while most of the florets still remained green in the treatment of AS6. With the treatment of AS6, ethylene production was reduced during the storage period, and the peak of ethylene production in AS6 treatment was significantly suppressed as compared with the control on the third day after harvest.
In addition, we found that the AS6 treatment upregulated the expression of BO-VTC1, BO-MDAR1, BO-MDAR2, and BO-DHAR, while downregulated the expression of BO-APX1, BO-APX2, and BO-sAPX. The higher expression levels of the AsA biosynthetic and regeneration genes, and the lower expression levels of the AsA oxidation genes led to the reduction of AsA loss in the AS6 treated florets after harvest.
The results presented in this study suggested that postharvest applications of AS6 were effective to delay the senescence of broccoli during the storage.
These results will provide new insights into maintaining the high quality of broccoli after harvest.
During senescence, the florets of broccoli turn yellow, and the contents of the bioactive compounds decrease to low levels.
The occurrence of senescence significantly reduces the nutritional and commercial values of broccoli.
In this study, to delay the senescence of broccoli, we treated the broccoli with a novel ABA receptor antagonist 3-hexylsulfanyl-ABA (AS6) after harvest at room temperature, and its effects on broccoli senescence were investigated.
The results showed that the yellowing process was delayed by the treatment of AS6. On the 4th day after harvest, the florets displayed obvious yellowing in the control, while most of the florets still remained green in the treatment of AS6. With the treatment of AS6, ethylene production was reduced during the storage period, and the peak of ethylene production in AS6 treatment was significantly suppressed as compared with the control on the third day after harvest.
In addition, we found that the AS6 treatment upregulated the expression of BO-VTC1, BO-MDAR1, BO-MDAR2, and BO-DHAR, while downregulated the expression of BO-APX1, BO-APX2, and BO-sAPX. The higher expression levels of the AsA biosynthetic and regeneration genes, and the lower expression levels of the AsA oxidation genes led to the reduction of AsA loss in the AS6 treated florets after harvest.
The results presented in this study suggested that postharvest applications of AS6 were effective to delay the senescence of broccoli during the storage.
These results will provide new insights into maintaining the high quality of broccoli after harvest.
Authors
G. Ma, L.C. Zhang, J. Takeuchi, Y. Todoroki, M. Kato
Keywords
ABA receptor antagonist, AsA, broccoli, florets, postharvest senescence, yellowing
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