Articles
Discovery of the satellite RNAs adapted to cucumber mosaic virus lily isolates
Article number
1392_1
Pages
1 – 6
Language
English
Abstract
Satellite RNAs (satRNAs) are dependent on their associated helper virus for both replication and encapsidation.
However, there are some reports on cucumber mosaic virus strains that cannot support satRNAs and it was reported that CMV lily isolates are unable to support the replication of satRNA in any of the host plants tested.
CMV lily isolates have a very narrow host range and are mainly spread in bulbs.
CMV (CMV-KL1 to CM-KL6) were isolated from virus-infected lilies but were confirmed to support satRNAs.
In addition, we found that CMV Ly2, isolated from lily, was found to support satellite RNA in Nicotiana benthamiana, increasing over serial passages.
Yamaguchi et al. (2005) identified that compatible amino acid residues at positions 876 and 891 of CMV-HL 1a protein were required for support of Y-sat RNA. Residue 876 of all CMV lily isolates examined (CMV-KL1 to CMV-KL6, CMV-Ly2 and CMV-HL) was shown to be Threonine.
Residue 891 of CMV-Ly2 was Leucine, as with CMV-Y, and CMV-KL1 to CMV-KL6 were all Valine, except for CMV-KL4, which was Methionine as in CMV-HL. Besides the residue 862 of all CMV lily isolates was identified as Arginine instead of Lysine.
These results suggest that residues 876 and 891 alone do not determine the compatibility between satRNAs and CMV. We also found that the symptom severity in N. benthamiana was very different in lily isolates with satRNAs.
As a result of the sequence alignment of satRNAs, it was confirmed that the sequence of Ly2-sat (severe mosaic) is clearly different from KL1-sat to KL6-sat (mild mosaic).
However, there are some reports on cucumber mosaic virus strains that cannot support satRNAs and it was reported that CMV lily isolates are unable to support the replication of satRNA in any of the host plants tested.
CMV lily isolates have a very narrow host range and are mainly spread in bulbs.
CMV (CMV-KL1 to CM-KL6) were isolated from virus-infected lilies but were confirmed to support satRNAs.
In addition, we found that CMV Ly2, isolated from lily, was found to support satellite RNA in Nicotiana benthamiana, increasing over serial passages.
Yamaguchi et al. (2005) identified that compatible amino acid residues at positions 876 and 891 of CMV-HL 1a protein were required for support of Y-sat RNA. Residue 876 of all CMV lily isolates examined (CMV-KL1 to CMV-KL6, CMV-Ly2 and CMV-HL) was shown to be Threonine.
Residue 891 of CMV-Ly2 was Leucine, as with CMV-Y, and CMV-KL1 to CMV-KL6 were all Valine, except for CMV-KL4, which was Methionine as in CMV-HL. Besides the residue 862 of all CMV lily isolates was identified as Arginine instead of Lysine.
These results suggest that residues 876 and 891 alone do not determine the compatibility between satRNAs and CMV. We also found that the symptom severity in N. benthamiana was very different in lily isolates with satRNAs.
As a result of the sequence alignment of satRNAs, it was confirmed that the sequence of Ly2-sat (severe mosaic) is clearly different from KL1-sat to KL6-sat (mild mosaic).
Authors
D.J. Min, J.S. Park, E.G. Song, K.H. Ryu, J.S. Hong
Keywords
plant disease, emergency, SatRNA detection, Lilium hybrids, RT-PCR
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