Articles
Survey and detection of virus and viroid diseases from chrysanthemum in Thailand
Article number
1392_3
Pages
15 – 24
Language
English
Abstract
Chrysanthemum plants (Chrysanthemum × morifolium) are susceptible to virus and viroid infections.
To identify causal viruses/viroids that induced symptoms on chrysanthemum, chrysanthemum leaves exhibiting virus- and viroid-like symptoms were surveyed and collected from cultivation areas in northern Thailand during 2019-2021 and subjected for detection of viruses and viroids.
Nine RNA viruses that were reported to infect chrysanthemum, including chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), chrysanthemum stem necrosis virus (CSNV), impatiens necrotic spot virus (INSV), tobacco mosaic virus (TMV), tomato aspermy virus (TAV), tomato spotted wilt virus (TSWV), turnip mosaic virus (TuMV), zucchini yellow mosaic virus (ZYMV) and two viroid including chrysanthemum chlorotic mottle viroid (CChMVd) and chrysanthemum stunt viroid (CSVd) were detected by using reverse transcription polymerase chain reaction (RT-PCR). Moreover, triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) was used to detect the DNA virus, tomato yellow leaf curl New Delhi virus (ToLCNDV), using the monoclonal antibody.
Results of virus and viroid detection from total of 223 samples revealed that 3 viruses were detected consisting of CVB, TMV and TuMV, and 2 viroids consisting of CChMVd and CSVd were detected by RT-PCR. Nucleotide sequences of detected viruses and viroids were analyzed and confirmed that they were actual pathogens compared to other isolates in GenBank.
Among all detected pathogens, CVB had the highest incidence.
Furthermore, the incidence of virus and viroid diseases in chrysanthemum of Thailand were lower than 10% according to detection results.
This study provided viral detection results along with associated symptoms observed on chrysanthemum cultivated in northern Thailand.
To identify causal viruses/viroids that induced symptoms on chrysanthemum, chrysanthemum leaves exhibiting virus- and viroid-like symptoms were surveyed and collected from cultivation areas in northern Thailand during 2019-2021 and subjected for detection of viruses and viroids.
Nine RNA viruses that were reported to infect chrysanthemum, including chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), chrysanthemum stem necrosis virus (CSNV), impatiens necrotic spot virus (INSV), tobacco mosaic virus (TMV), tomato aspermy virus (TAV), tomato spotted wilt virus (TSWV), turnip mosaic virus (TuMV), zucchini yellow mosaic virus (ZYMV) and two viroid including chrysanthemum chlorotic mottle viroid (CChMVd) and chrysanthemum stunt viroid (CSVd) were detected by using reverse transcription polymerase chain reaction (RT-PCR). Moreover, triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) was used to detect the DNA virus, tomato yellow leaf curl New Delhi virus (ToLCNDV), using the monoclonal antibody.
Results of virus and viroid detection from total of 223 samples revealed that 3 viruses were detected consisting of CVB, TMV and TuMV, and 2 viroids consisting of CChMVd and CSVd were detected by RT-PCR. Nucleotide sequences of detected viruses and viroids were analyzed and confirmed that they were actual pathogens compared to other isolates in GenBank.
Among all detected pathogens, CVB had the highest incidence.
Furthermore, the incidence of virus and viroid diseases in chrysanthemum of Thailand were lower than 10% according to detection results.
This study provided viral detection results along with associated symptoms observed on chrysanthemum cultivated in northern Thailand.
Authors
S. Supakitthanakorn, O. Himananto, O. Ruangwong
Keywords
CVB, CChMVd, CSVd, TMV, TuMV, RT-PCR, TAS-ELISA
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