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DETECTION OF STRAWBERRY MILD YELLOW EDGE VIRUS BY SEROLOGICALLY SPECIFIC ELECTRON MICROSCOPY
However, its serological relationship to other members of this group has not been pregiously demonstrated.
In preliminary experiments, partially purified preparations from infected plant material were examined for the presence of virus particles by the dip method.
A few virus particles in groups of two or three were found in rather low concentration (1–2 groups per hole using a 300 mesh grid). When this preparation was reacted with an antiserum made against beet wetern yellows virus (BWYV) (
-globulin fraction, lmg/ml, diluted 1:250 in 0.1M phosphate buffer, pH 7) trapping occured: 15–25 particles per field of view were easily observed at a magnification of 25000x.
However, no decoration was obtained with this BWYV antiserum, even when used at a dilution of 1:10.
Since BMYV antisrum reacts with many members of the luteovirus group, this finding indicates that SMYEV may indeed be a luteovirus related closely enough to BWYV to be trapped by SSEM, but too distant for decoration with the BWYV antiserum.