Articles
OCCURENCE OF RASPBERRY BUSHY DWARF VIRUS IN NATIVE THIMBLEBERRY IN BRITISH COLUMBIA
Article number
186_2
Pages
17 – 22
Language
Abstract
During a survey of native Rubus species in British Columbia for the possible presence of viruses, some plants of thimbleberry (R. parviflorus) were observed showing viruslike symptoms.
Young expanding leaves from these plants were sap-inoculated to a range of herbaceous hosts, one of which, Chenopodium quinoa, developed characteristic mottling symptoms.
This host was used for propagation and purification of the causal virus.
Density gradient scans indicated that this was a single-component virus, and the particles comprising the peak were quasi-isometric spheres 30 nm in diameter.
In serological tests with antisera against a range of spherical plant viruses, the thimbleberry virus was shown to be related to raspberry bushy dwarf virus (RBDV). A polyclonal antiserum was prepared by injecting the purified particles into rabbits and a monoclonal antibody was obtained in mice.
In aqar gel serology tests the thimbleberry isolate was serologically indistinguishable from an isolate obtained from raspberry.
A survey of thimbleberry plants in the Fraser Valley area of British Columbia indicated that the virus is widespread, even in areas remote from small fruit cultivation.
Of 570 plants indexed by ELISA, 109 (19%) were infected; the survey results indicate that RBDV is endemic in thimbleberry and the virus source is not chance contamination from infected red raspberry.
In graft-transmission tests, the virus was transmitted to several R. idaeus cultivars, and to R. henryi, R. occidentalis and Fragaria vesca cv.
Alpine.
Red raspberry cultivars resistant to the raspberry isolate of RBDV were also shown to be resistant to the isolate from thimbleberry.
The virus was seed-transmitted in Alpine strawberry and thimbleberry.
Young expanding leaves from these plants were sap-inoculated to a range of herbaceous hosts, one of which, Chenopodium quinoa, developed characteristic mottling symptoms.
This host was used for propagation and purification of the causal virus.
Density gradient scans indicated that this was a single-component virus, and the particles comprising the peak were quasi-isometric spheres 30 nm in diameter.
In serological tests with antisera against a range of spherical plant viruses, the thimbleberry virus was shown to be related to raspberry bushy dwarf virus (RBDV). A polyclonal antiserum was prepared by injecting the purified particles into rabbits and a monoclonal antibody was obtained in mice.
In aqar gel serology tests the thimbleberry isolate was serologically indistinguishable from an isolate obtained from raspberry.
A survey of thimbleberry plants in the Fraser Valley area of British Columbia indicated that the virus is widespread, even in areas remote from small fruit cultivation.
Of 570 plants indexed by ELISA, 109 (19%) were infected; the survey results indicate that RBDV is endemic in thimbleberry and the virus source is not chance contamination from infected red raspberry.
In graft-transmission tests, the virus was transmitted to several R. idaeus cultivars, and to R. henryi, R. occidentalis and Fragaria vesca cv.
Alpine.
Red raspberry cultivars resistant to the raspberry isolate of RBDV were also shown to be resistant to the isolate from thimbleberry.
The virus was seed-transmitted in Alpine strawberry and thimbleberry.
Authors
R. Credi, J. L. Shier, R. Stace-Smith
Keywords
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