Articles
EFFECT OF GAMMA RADIATIONS, ETHYL METHANE SULFONATE AND SODIUM AZIDE ON THE SENNOSIDE CONTENT IN CASSIA ANGUSTIFOLIA
Article number
208_24
Pages
231 – 236
Language
Abstract
Cassia angustifolia commonly known as Tinnevelly Senna is an excellent source of sennosides.
The leaflets of this plant constitute the principal raw material.
To investigate the effect(s) of mutagenic treatments on phytochemical characteristics, the sand treated dry seeds of Senna were treated with different doses of gamma radiations, ethyl methane sulfonate and sodium azide for 18 hours duration at 24 ± 2°C. The treated population in M1 generation exhibited different degree of stimulation in growth.
Other parameters were also affected by all the three mutagenic treatments.
Seeds collected from M1 generation were sown and M2 plant progenies were raised.
Morphological mutations with desirable characters were further investigated.
Biochemical estimation of sennoside from the leaflets in vegetative and blooming phases was carried out.
Of the three mutagens, 0.006% sodium azide yielded maximum sennoside in the vegetative phase; while, in the blooming phase, it was highest with 20 kR of gamma radiations.
Seeds of these plant types hold promise for enhancing the sennoside yields per gram of tissue.
The leaflets of this plant constitute the principal raw material.
To investigate the effect(s) of mutagenic treatments on phytochemical characteristics, the sand treated dry seeds of Senna were treated with different doses of gamma radiations, ethyl methane sulfonate and sodium azide for 18 hours duration at 24 ± 2°C. The treated population in M1 generation exhibited different degree of stimulation in growth.
Other parameters were also affected by all the three mutagenic treatments.
Seeds collected from M1 generation were sown and M2 plant progenies were raised.
Morphological mutations with desirable characters were further investigated.
Biochemical estimation of sennoside from the leaflets in vegetative and blooming phases was carried out.
Of the three mutagens, 0.006% sodium azide yielded maximum sennoside in the vegetative phase; while, in the blooming phase, it was highest with 20 kR of gamma radiations.
Seeds of these plant types hold promise for enhancing the sennoside yields per gram of tissue.
Authors
A.S. Khalatkar, Y.R. Bhargava
Keywords
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