Articles
RAPID PROPAGATION OF FRENCH TARRAGON USING IN VITRO TECHNIQUES
Article number
208_27
Pages
251 – 262
Language
Abstract
In vitro conditions were examined in order to maximize axillary shoot proliferation of French tarragon, Artemisia dracunulus L. var sativa. Basal medium consisted of full strength Murashige-Skoog salts with the following addenda per liter: sucrose, 20 g; pyridoxine· HCl, 0.5 mg; thiamine.HCl, 0.1 mg; nicotinic acid, 0.5 mg; myo-inositol, 100 mg; and glycine, 2 mg; and was supplemented with benzyladenine (BA) and naphthaleneacetic acid (NAA) or 2,4-dicholorophenoxyacetic acid (2,4-D). Shoots proliferated optimally in the presence of 0.4 mg/liter BA in combination with 0.05 mg/liter NAA. Other factors examined were shoot orientation in vitro, apical pinching, apical versus axillary shoot-tip explants and gelling agent.
Horizontally placed shoot-tip explants produced more axillary shoots than vertically placed explants.
There is an inverse relationship between proliferation and the concentration of washed Difco Bacto agar or Gelrite®, except at the lowest Gelrite concentration (0.5%) where shoots became vitrified.
Horizontally placed shoot-tip explants produced more axillary shoots than vertically placed explants.
There is an inverse relationship between proliferation and the concentration of washed Difco Bacto agar or Gelrite®, except at the lowest Gelrite concentration (0.5%) where shoots became vitrified.
Authors
W.A. Mackay, S.L. Kitto
Keywords
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