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Articles

PRACTICAL ASPECTS IN THE PRODUCTION AND USE IN ELISA OF ANTISERA AGAINST VIRUSES IN BULBOUS CROPS

Article number

234_21

Pages

185 – 190

Language

Abstract

Over a period of ten years many antisera to viruses in bulbous crops have been prepared for use in ELISA. These antisera were mainly destined for use by inspection services (Derks, 1988). We have looked for suitable immunization and bleeding schedules for large-scale production of antisera.
In addition, methods of y-globulin purification and conjugation were compared.
In this article a summary is given of the results of these experiments.

Publication

VII International Symposium on Virus Diseases of Ornamental Plants

Authors

A.F.L.M. Derks, M.E.C. Lemmers, C.I.M. van der Vlugt

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1988.234.21

Online Articles (65)

2. NEW VIRUSES AND VIRUS STRAINS

R.H. Lawson

3. RAPID DIAGNOSIS OF ORNITHOGALUM AND LACHENALIA VIRUSES IN PROPAGATION STOCK

J.T. Burger | M.B. von Wechmar

4. A POTYVIRUS INFECTING RANUNCULUS ASIATICUS

M.S. Elliott | M. Gallegati | F.W. Zettler | N.-J. Ko

5. SOME PROPERTIES OF A VIRUS ISOLATED FROM CASSIA CORYMBOSA WITH A MOSAIC DISEASE

N. Ngamyeesoon | R.G.T. Hicks

6. VIRUSES IN DIPLADENIA SANDERI

N. Paludan | M. Heide | J. Begtrup | B. Borkhardt

7. A STRAIN OF CLOVER YELLOW VEIN VIRUS ISOLATED FROM CALANTHE SP.

N. Inouye | T. Maeda | K. Mitsuhata

8. OCCURRENCE OF CANNA YELLOW MOTTLE VIRUS IN NORTH AMERICA

B.E.L. Lockhart

9. PURIFICATION AND MECHANICAL TRANSMISSION OF KALANCHOE TOP-SPOTTING-ASSOCIATED VIRUS

B.E.L. Lockhart | Z. Ferji

10. VIROIDS AND VIROID DISEASES

Heinz L. Sänger

11. THE EFFECT OF TEMPERATURE AND LIGHT ON CHRYSANTHEMUM STUNT VIROID INFECTION OF FLORISTS CHRYSANTHEMUM

M.K. Handley | R.K. Horst

12. REDUCED SUSCEPTIBILITY OF FLORISTS’ CHRYSANTHEMUM TO ERWINIA CHRYSANTHEMI FOLLOWING VIROID INFECTION

R.J. McGovern | R.K. Horst | R.S. Dickey

13. INFECTIVITY OF THE VIROID-LIKE RNAS ASSOCIATED WITH THE CARNATION STUNTING SYNDROME

G. Boccardo | M. d'Aquilio | P. Palukaitis | C. Marzachi | V. Lisa

14. CLONED NUCLEIC ACID HYBRIDIZATION PROBES IN DETECTION AND CLASSIFICATION OF MYCOPLASMALIKE ORGANISMS (MLOS)

Robert E. Davis | I. Lee | Ellen L. Dally | N. Dewitt | Sharon M. Douglas

15. PHYLLODY AND VIRESCENCE IN RANUNCULUS HYBRIDS

A. Bertaccini | F. Marani | S. Rapetti

16. SOME CHARACTERISTICS OF CHRYSANTHEMUM YELLOWS DISEASE

M. Conti | G. D'Agostino | A. Casetta | L. Mela

17. MYCOPLASMA DISEASES OF DIMORPHOTHECA SINUATA AND CATHARANTHUS ROSEUS IN SICILY

V. Grimaldi | S. Grasso

18. CHRYSANTHEMUM PHLOEM NECROSIS: MICROSCOPY OF THE PUTATIVE PATHOGEN

19. EFFECTS OF TEMPERATURE ON EXPRESSION OF CHRYSANTHEMUM SLOW DECLINE IN CHRYSANTHEMUM MORIFOLIUM

R.H. Lawson | H.T. Hsu

20. VIRUS DISEASES IN THE GENT REGION

W. Welvaert | G. Samyn

21. RECENT ADVANCES IN SERODIAGNOSIS OF PLANT VIRUS DISEASES

22. PRACTICAL ASPECTS IN THE PRODUCTION AND USE IN ELISA OF ANTISERA AGAINST VIRUSES IN BULBOUS CROPS

A.F.L.M. Derks | M.E.C. Lemmers | C.I.M. van der Vlugt

23. TOWARDS A RAPID AND RELIABLE DETECTION METHOD FOR IRIS SEVERE MOSAIC VIRUS IN IRIS BULBS

C. I.M. van der Vlugt | A.F.L.M. Derks | J. Dijkstra | R. Goldbach

24. DETECTION OF IRIS MILD MOSAIC VIRUS IN BULBS AND LEAVES OF BULBOUS IRIS BY ELISA

A.R. van Schadewijk | A.F.L.M. Derks | M.E.C. Lemmers | Th.C. Hollinger

25. AN APPRAISAL OF THE IDENTITIES OF POTYVIRUSES INFECTING NARCISSUS

W.P. Mowat | G.H. Duncan | S. Dawson

26. IMMUNOLOGICAL DETECTION OF CARNATION YELLOW FLECK VIRUS (CYFV) IN INFECTED CARNATIONS

C. PONCET | J.C. DEVERGNE

27. ELISA DETECTION OF CARNATION VEIN MOTTLE AND CARNATION LATENT VIRUSES

E. Luisoni | M. Vecchiati | P. Roggero

28. OCCURENCE AND IDENTIFICATION OF CARNATION NECROTIC FLECK VIRUS IN CZECHOSLOVAKIA

V. Mokrá | J. Mertelík | B. Götzová

29. NATURAL INFECTION OF TULIPS WITH TURNIP MOSAIC VIRUS AND ANOTHER POTYVIRUS ISOLATE DISTINCT FROM TULIP BREAKING VIRUS IN THE U.S.A.

J. Hammond | Gary A. Chastagner

30. ELISA INDEXING OF ODONTOGLOSSUM RINGSPOT VIRUS USING A UREASE-ANTIBODY CONJUGATE AND ITS USE IN FIELD SURVEYS

P.R. Beetham | J.R. Moran

31. COMPARISON OF ANTIGEN AND ANTIBODY-COATED ENZYME-LINKED IMMUNOSORBENT ASSAY PROCEDURES FOR THE DETECTION OF THREE ISOLATES OF PURIFIED APPLE MOSAIC VIRUS OR PRUNUS NECROTIC RINGSPOT VIRUS

S.-M. Wong | R.K. Horst

32. COMPLEMENTARY NUCLEIC ACIDS AND ANTIBODIES IN PLANT VIRUS DIAGNOSIS

R. Koenig

33. SENSITIVE AND SPECIFIC DETECTION OF TWO FILAMENTOUS VIRUSES FROM NERINES USING CLONED CDNA PROBES

G. Balasingam | N. Ellison | K.S. Milne | R.L.S. Forster

34. DETECTION OF VIRUSES IN GLADIOLI CORMS

A. Stein | S. Levy | G. Loebenstein

35. THE DETECTION OF DASHEEN MOSAIC VIRUS USING THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

B.C. Rodoni | J.R. Moran

36. RECENT ASPECTS OF THE USE OF CYTOPATHOLOGY IN VIRUS IDENTIFICATION

D.-E. Lesemann

37. IMMUNOCYTOCHEMICAL IDENTIFICATION OF CYMBIDIUM MOSAIC AND ODONTOGLOSSUM RINGSPOT VIRUSES IN ORCHID TISSUES

N.-J. Ko | F.W. Zettler | J.R. Edwardson

38. INVESTIGATION OF THE ETIOLOGY AND CYTOLOGY OF CHRYSANTHEMUM SLOW DECLINE

M.M. Dienelt | R.H. Lawson

39. CYTOPATHIC STRUCTURES ASSOCIATED WITH ROBINIA MOSAIC VIRUS, A STRAIN OF PEANUT STUNT VIRUS

Z. Štefanac | M. Wrischer | N. Bezic

40. QUANTITATIVE USE OF THE ELECTRON MICROSCOPE DECORATION TECHNIQUE FOR PLANT VIRUS DIAGNOSTICS

R.G. Milne

41. NEW POTENTIAL QUARANTINE PROBLEMS FOR ORNAMENTALS IN EUROPE

O. Lovisolo

42. EPIDEMIOLOGY – ITS SCOPE AND VALUE

R.G. Garrett

43. ECOLOGY OF VIRUSES IN ORNAMENTAL AND FOREST TREES

J.I. Cooper

44. A STRAIN OF BEAN YELLOW MOSAIC VIRUS IS APHID-TRANSMITTED FROM ORCHID

J. Hammond | R.H. Lawson

45. THE ROLE OF WEEDS IN THE EPIDEMIOLOGY OF GLADIOLUS VIRUSES AND MLO

V. Vicchi | M.G. Bellardi

46. NATURALLY OCCURRING VIRUSES OF POKEWEED FOUND IN SOUTH CAROLINA

J. Xia | S.W. Scott | O.W. Barnett

47. OCCURRENCE OF CAMELLIA LEAF YELLOW MOTTLE VIRUS (CLYMV) ON EAST ADRIATIC COAST

M. GAILHOFER | I. THALER | D. MILICIC

48. PRODUCTION OF PLANTS FREE OF VIRUS AND PREVENTION OF REINFECTION

R.K. Horst

49. RESISTANCE RESPONSES OF PLANTS TO VIRUS INFECTIONS

G. Loebenstein | A. Gera

50. ERADICATION OF ORCHID VIRUSES BY CHEMOTHERAPY FROM IN-VITRO CULTURES OF CYMBIDIUM

J. ALBOUY | C. FLOUZAT | C. KUSIAK | M. TRONCHET

51. PRODUCTION OF VIRUS-FREE DAHLIA BY: MERISTEM-CULTURE AND VIRUS DETECTION THROUGH CDNA PROBES AND ELISA

W. CAI | M. TRONCHET | N. LARROQUE | N. DORION | J. ALBOUY

52. THE EFFECTS OF PRUNUS NECROTIC RINGSPOT VIRUS ON PRODUCTION AND QUALITY OF ROSE FLOWERS

J.R. Moran | J.D. Faragher | D.M. Baker

53. EFECTS OF PRUNUS NECROTIC RINGSPOT VIRUS ON GRAFT UNION AND FLOWER QUALITY OF GLASSHOUSE GROWN ROSES IN ISRAEL

A. Stein | S. Levy | G. Loebenstein

54. SYMPTOMATOLOGY AND OCCURRENCE OF APPLE MOSAIC AND PRUNUS NECROTIC RINGSPOT VIRUSES ON ROSE IN NEW YORK

S.-M. Wong | R.K. Horst | R.W. Langhans

55. PRESENT STATUS OF THE STUDIES ON ORNAMENTAL PLANT VIRUS DISEASES IN BRAZIL

E.W. Kitajima

56. NARCISSUS MOSAIC VIRUS IN LILY

M.G. Bellardi | F. Marani | A. Bertaccini

57. CUCUMBER MOSAIC VIRUS IN LILIES IN ITALY

M.P. Benetti | L. Tomassoli

58. DIFFERENCES IN PARTICLE STABILITY AND ELECTROPHORETIC BEHAVIOUR AMONG TOMATO ASPERMY VIRUS ISOLATES

M. Plazibat | Z. Štefanac

59. FURTHER INFORMATION ON INFECTION OF IMPATIENS HOLSTII WITH HELENIUM VIRUS S

N. Pleše | Z. Eric | M. Krajacic

60. SUSCEPTIBILITY OF SOME ORNAMENTAL PLANTS TO MELANDRIUM YELLOW FLECK VIRUS, A NEW MEMBER OF BROMOVIRUS GROUP

J. Horváth | E. Ladányi | M. Hollings

61. VIRUS DAMAGE EVALUATION

O.W. Barnett

62. NEW PERSPECTIVES FOR CONTROL OF PLANT VIRUS DISEASES OPENED BY BIOTECHNOLOGY

L. Vloten-Doting

63. NEW ECONOMICALLY-IMPORTANT VIRUS AND VIRUS-LIKE DISEASES OF ORNAMENTAL PLANTS

A.A. Brunt

64. DIAGNOSIS IN COMMERCIAL PRACTICE WITH SPECIAL REFERENCE TO ORNAMENTAL CROPS

A.F.L.M. Derks

65. PRODUCTION AND TRADE OF HEALTHY PROPAGATING MATERIAL : A NEED FOR A QUALITY CONTROL SYSTEM

J.E.M. van Ruiten