Articles
SOME PROPERTIES OF A VIRUS ISOLATED FROM CASSIA CORYMBOSA WITH A MOSAIC DISEASE
Article number
234_4
Pages
45 – 52
Language
Abstract
A virus isolated from a mature ornamental Cassia corymbosa Lam plant was readily sap-transmitted to a moderate range of herbaceous indicators.
The virus was relatively unstable in sap extracts of Nicotiana clevelandii, with a thermal inactivation point of 50–55°C, a dilution end point of 10-4 – 10-5, and a longevity in vitro at room temperature, of less than 24 h; sap stored at -20°C was still infective after 10 months.
Examination of leaf ‘squashes’ in the electron microscope showed flexuous, filamentous particles, about 150nm to 2 000nm long, but with no clear modal length.
Analysis of nucleic acid isolated from infected C. c o rymbosa revealed at least two bands of dsRNA, the largest of which had an estimated molecular weight of 13–15 x 106.
The virus was relatively unstable in sap extracts of Nicotiana clevelandii, with a thermal inactivation point of 50–55°C, a dilution end point of 10-4 – 10-5, and a longevity in vitro at room temperature, of less than 24 h; sap stored at -20°C was still infective after 10 months.
Examination of leaf ‘squashes’ in the electron microscope showed flexuous, filamentous particles, about 150nm to 2 000nm long, but with no clear modal length.
Analysis of nucleic acid isolated from infected C. c o rymbosa revealed at least two bands of dsRNA, the largest of which had an estimated molecular weight of 13–15 x 106.
A single lesion isolate of the Cassia virus was mechanically inoculated to apparently virus-free, and dsRNA-free, seedlings of C. corymbosa, two of which developed symptoms similar to those on the original shrub.
A virus apparently identical in host reaction and particle morphology, and with similar dsRNA bands, to the Cassia virus, was isolated from inoculated seedlings.
Authors
N. Ngamyeesoon, R.G.T. Hicks
Keywords
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