Articles
OCCURRENCE OF SEED-TRANSMISSIBLE DOUBLE-STRANDED RNA IN NATIVE RED AND BLACK RASPBERRY
Article number
236_1
Pages
13 – 20
Language
Abstract
Extraction of double-stranded RNA (dsRNA) from several native Rubus spp. revealed the presence of dsRNA in two species, R. leucodermis and R. strigosus. Some plants in both species showed a similar dsRNA pattern of a major band of Mr 3.4 x 106. When this band was particularly prominent, a minor band of Mr 1.7 x 106 was also evident.
The major band and occasionally the minor band were detected in 30 of 52 R. leucodermis and 5 of 18 R. strigosus plants that were assayed.
From 4 to 92 percent of seed collected from R. leucodermis plants with these bands produced seedlings that contained bands.
Another dsRNA pattern, consisting of two bands of Mr 1.12 x 106 and 1.03 x 106 was observed in 10 to 17 percent of seedlings from three R. leucodermis plants.
This doublet is distinct from the two bands described above, and has not been seen in conjunction with them in the same plant.
Neither of these dsRNA patterns has been found in any cultivated red raspberry (R. idaeus) plants.
None of the suspect R. leucodermis or R. strigosus plants showed symptoms, although the dsRNA patterns in this molecular weight range are indicative of virus infection.
Thin section electron microscopy of leaf tissue showed no evidence of virus particles.
Random primed cDNA made from the major dsRNA band hybridized to total DNA isolated from R. leucodermis and from R. idaeus.
The major band and occasionally the minor band were detected in 30 of 52 R. leucodermis and 5 of 18 R. strigosus plants that were assayed.
From 4 to 92 percent of seed collected from R. leucodermis plants with these bands produced seedlings that contained bands.
Another dsRNA pattern, consisting of two bands of Mr 1.12 x 106 and 1.03 x 106 was observed in 10 to 17 percent of seedlings from three R. leucodermis plants.
This doublet is distinct from the two bands described above, and has not been seen in conjunction with them in the same plant.
Neither of these dsRNA patterns has been found in any cultivated red raspberry (R. idaeus) plants.
None of the suspect R. leucodermis or R. strigosus plants showed symptoms, although the dsRNA patterns in this molecular weight range are indicative of virus infection.
Thin section electron microscopy of leaf tissue showed no evidence of virus particles.
Random primed cDNA made from the major dsRNA band hybridized to total DNA isolated from R. leucodermis and from R. idaeus.
Authors
R. Stace-Smith, Robert R. Martin
Keywords
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