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Articles

CRYOPRESERVATION OF LILY MERISTEMS

Article number

266_43

Pages

331 – 338

Language

Abstract

Cryopreservation may become an important tool for long term conservation of valuable lily cultivars.
We selected meristems for cryopreservation because of their genetic stability upon regrowth in normal tissue culture.
The excised meristems only survived cryopreservation after a preculture at 5°C for at least 5 days.
Preculture in the presence of dimethylsulfoxide (DMSO) followed by a direct plunge into liquid nitrogen or preculture without DMSO followed by a short treatment with cryoprotectants (DMSO and/or glycerol) and slow freezing, resulted in survival percentages of 8% or less.

Publication

V International Symposium on Flower Bulbs

Authors

H. Bouman, G.J. de Klerk

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.266.43

Online Articles (78)

1. HISTORY OF BULB GROWING IN WASHINGTON STATE

C. J. Gould

2. PRODUCTION AND MAINTENANCE OF VIRUS-FREE BULBS

R.H. Lawson

3. RECENT ADVANCES IN CONTROL OF FLOWERING AND GROWTH HABIT OF GEOPHYTES

A. H. Halevy

4. PERSPECTIVES ON FUTURE MARKETING, RESEARCH, AND EDUCATIONAL PROGRAMS FOR FLOWER BULBS

A.A. De Hertogh

5. THE DEVELOPMENT OF DORMANCY IN LILY BULBLETS GENERATED IN VITRO

A.M.G. Paffen | P. Aguettaz | I. Delvallée | G.J. de Klerk | R.J. Bogers

6. ROLE OF OUTER SCALE IN TWIN-SCALE PROPAGATION OF HIPPEASTRUM HYBRIDUM AND COMPARISON OF BULBLET FORMATION FROM SINGLE- AND TWIN-SCALES

H. Okubo | C.W. Huang | S. Uemoto

7. POTENTIAL AND LIMITATIONS OF NARCISSUS MICROPROPAGATION: AN EXPERIMENTAL EVALUATION

W.M. Squires | F.A. Langton

8. IN VITRO BULBLET PROPAGATION OF VIRUS-FREE DUTCH IRIS.

Wilbur C. Anderson | K. A. Mielke | P. N. Miller | T. Allen

9. COMPARISONS OF PRECULTURAL TREATMENTS AND CULTURAL CONDITIONS ON IN VITRO RESPONSE OF TULIP

C.M. Baker | H.F. Wilkins | P.D. Ascher

10. INFLUENCE OF CULTURE ENVIRONMENT ON SHOOT GROWTH AND BULBING OF TULIP IN VITRO

P.G. Alderson | A.G. Taeb

11. LOCALIZATION OF RESERVE MOBILIZATION DURING SCALET FORMATION ON EASTER LILY SCALES

W. B. Miller

12. EFFECTS OF STORAGE, COOLING AND GREENHOUSE CONDITIONS ON ANEMONE BLANDA, FRITILLARIA MELEAGRIS AND OXALIS ADENOPHYLLA FOR USE AS POTPLANT.

P.J. van Leeuwen | A.J. Dop

13. YEAR-ROUND CULTURE OF TULIPS

K.Y. de Jong | F.Th. de Greef | A.J. Dop | N.A.A. Hof

14. FORCING DUTCH-GROWN FREESIAS AS POTTED PLANTS IN THE U.S. AND CANADA

A.A. De Hertogh | R. Milks

15. GROWTH AND DEVELOPMENT OF HIPPEASTRUM GROWN IN GLASSHOUSES

J.C. Doorduin

16. RESPIRATION AND ETHYLENE EVOLUTION IN RELATION TO FLOWER BUD ABSCISSION IN ASIATIC HYBRID LILY

Mark S. Roh

17. EFFECT OF HIGH TEMPERATURE ON BUD BLAST IN ASIATIC HYBRID LILY

Mark S. Roh

18. BUD ABNORMALITIES DURING YEAR-ROUND FORCING OF ASIATIC HYBRID LILIES

Mark S. Roh

19. EFFECT OF STARTING DATE AND TEMPERATURE OF STORAGE ON CHANGES IN FREE AMINO ACIDS IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN, AS RELATED TO THE SPECIAL PRECOOLING

N. Gorin | J. Tonecki | J.M. Franssen

20. STARCH SYNTHASE IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN STORED AT 5 OR 17 °C

N. Gorin | F.G. van Dam

21. THE EFFECT OF AIR AND SOIL TEMPERATURE ON ASSIMILATE PARTITIONING AND FLOWER BUD INITIATION OF FREESIA.

J. Berghoef | A.P. Zevenbergen

22. THE DEVELOPMENT OF FLOWERING ASSAYS FOR COLD-TREATED TULIP BULBS

P.M. Boonekamp | J.C.M. Beijersbergen | J.M. Franssen

23. FLOWER INITIATION IN THREE ASIATIC HYBRID LILY CULTIVARS

X. Zhang | D.J. Beattie | J.W. White

24. EFFECTS OF LOW LIGHT INTENSITY AND LOW TEMPERATURE GIVEN AT DIFFERENT DEVELOPMENTAL STAGES ON FLOWERING OF GLADIOLUS

H. Imanishi | Y. Imae

25. GROWTH AND LEAF PHOTOSYNTHESIS OF LILIUM LONGIFLORUM THUNB. ‘NELLIE WHITE’ IN RESPONSE TO PARTIAL DEFOLIATION AFTER ANTHESIS

Y. Wang

26. GROWTH RETARDANTS FOR FLOWERING POT PLANT PRODUCTION OF NARCISSUS ‘GRAND SOLEIL D’OR’

H. Yahel | D. Ziv | M. Ron

27. TIME OF FLOWER BUD DIFFERENTIATION IN ASIATIC HYBRID LILIES

K. Ohkawa | A. Kano | A. Nukaya

28. HISTOCHEMICAL TEST FOR PRESENCE OR ABSENCE OF CHALCONES IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN PRECOOLED AT 5 °C OR KEPT AT 17 °C

N. Gorin | R. Sütfeld | J. Tonecki | J.H. Franssen | N. Haanappel

29. CHANGES IN RESPIRATORY CHARACTERISTICS AND ETHYLENE PRODUCTION IN TULIP BULBS AFTER COLD TREATMENT

W.A. Kanneworff | L.H.W. van der Plas

30. EFFECTS OF ETHYLENE ACTION INHIBITORS AND ACC ON INDUCTION OF FLOWER BUDS OF DUTCH IRIS CV. BLUE MAGIC

D. Yue | H. Imanishi

31. THE EFFECTS OF PHOTOPERIOD, PPF, PROMALIN, STS AND WATER ON FLOWERING OF ASIATIC HYBRID LILIES

X. Zhang | J.W. White | D.J. Beattie

32. THE EFFECT OF PRECOOLING, ENVIRONMENTAL FACTORS AND GROWTH-REGULATING SUBSTANCES ON PLANT HEIGHT OF FREESIA AS POTPLANT

J. Berghoef | A.P. Zevenbergen

33. ‘TÊTE À TÊTE’, A BEAUTIFUL BUT DELICATE POT NARCISSUS

P.J.M. Vreeburg | J.A. Schipper

34. CULTURE OF NARCISSUS TAZETTA ‘ZIVA’ IN THE NETHERLANDS

P.J.M. Vreeburg | A.J. Dop

35. VARIETY EVALUATION OF HIPPEASTRUM

ir. A. de Gelder

36. PALE FLOWERS IN IRIS CV. ‘IDEAL’

J.A. Schipper | G.G.M. van der Valk

37. RELATIONS BETWEEN QUALITY AND ORIGIN OF FORCING BULBS, FORCING METHODS AND THE ORNAMENTAL VALUE OF FORCED TULIPS

B.M.M. Kortekaas | C.O.N. de Vroomen

38. POST-HARVEST VARIATION OF FREE ARGININE IN BASAL PLATE TISSUES OF TULIP BULBS ; RELATION TO BULB PHYSIOLOGICAL EVOLUTION

M. Le Nard | V. Fiala

39. THE EFFECT OF BASAL-PLATE TISSUE ON THE KEEPING-QUALITY OF HYACINTH CUT FLOWERS

A. Swart

40. MODE OF ACTION OF METAL SALTS AND SUCROSE EXTENDING THE VASE LIFE OF CUT GLADIOLI

T.P. Murali

41. OVERCOMING INTERSPECIFIC CROSSING BARRIERS IN LILIUM BY OVARY AND EMBRYO CULTURE

Jaap M. van Tuyl | K. Sande | R. Diën | D. Straathof | Hein M.C. van Holsteijn

42. RESEARCH ON POLYPLOIDY IN INTERSPECIFIC HYBRIDIZATION OF LILY

J.M. van Tuyl | H.M.C. van Holsteijn | A.A.M. Kwakkenbos

43. CRYOPRESERVATION OF LILY MERISTEMS

H. Bouman | G.J. de Klerk

44. GENETIC IMPROVEMENT OF LILIUM IN ITALY

A. Grassotti | F. Torrini | A. Mercuri | T. Schiva

45. THE BREEDING OF INTERSPECIES AND INTERGENERA HYBRIDS IN THE AMARYLLIDACEAE

A.F. Coertze | E. Louw

46. PROSPECTS OF INTERSPECIFIC HYBRIDIZATION IN DUTCH IRIS

W. Eikelboom | J.P. van Eijk

47. EVALUATION OF BREEDING RESEARCH ON RESISTANCE TO FUSARIUM OXYSPORUM IN TULIP

J.P. van Eijk | W. Eikelboom

48. DEVELOPING A TEST METHOD FOR SELECTION ON RESISTANCE TO DRY-ROT DISEASE (STROMATINIA GLADIOLI) IN GLADIOLUS

J.P. van Eijk | A. van Zaayen | W. Eikelboom

49. NITROGEN-FERTILIZER RECOMMENDATIONS FOR GLADIOLUS BASED ON N-MINERAL SOIL ANALYSIS

N.P.A. Groen | J.H.G. Slangen

50. CULTURE AND LIFTING OF FLOWERBULBS ON HEAVY-TEXTURED SOILS

R.S. Bijl

51. CHANGING VIEWS ON CHEMICAL CROP PROTECTION IN THE NETHERLANDS AND THE CONSEQUENCES FOR BULB RESEARCH

J. van Aartrijk | M. de Rooy

52. STRUCTURE AND APPLICATIONS OF A PRODUCTION MODEL IN TULIP BULB CULTURE

G.G.M. van der Valk | J.B.H.M. van Gils

53. ADVISORY SERVICE FOR PURCHASERS OF FLOWER BULBS OUTSIDE THE NETHERLANDS

J.C.M. Buschman

54. CONTROL OF FUSARIUM DISEASE IN IXIA BY DIPPING PROPAGATION MATERIAL

C. Pasini | M.G. Fantino

55. THE INHIBITORY ACTION IN VITRO OF DIETHOFENCARB AGAINST PATHOGENIC FUNGI FROM BULBOUS AND TUBEROUS PLANTS

P. Vink | A.Th.J. Koster

56. CONTROL OF RHIZOCTONIA SOLANI IN THE FIELD WITH A LOW DOSAGE OF TOLCLOFOS-METHYL OR FLUTOLANIL

A.Th.J. Koster | L.J.v.d. Meer

57. PRELIMINARY RESULTS ON BIOLOGICAL CONTROL OF BOTRYTIS CINEREA IN FORCED TULIPS

M.G. Pennock-Vos | E.J.A. Roebroeck | Cz. Skrzypczak

58. RESISTANCE OF BOTRYTIS ELLIPTICA TO FUNGICIDES

Q. Migheli | C. Aloi | M.L. Gullino

59. OCCURRENCE AND CONTROL OF BENZIMIDAZOLE AND DICARBOXIMIDE RESISTANT BOTRYTIS SPP. ON BULB CROPS IN WESTERN WASHINGTON AND OREGON

Gary A. Chastagner | K. Riley

60. BOTRYTIS CINEREA, CAUSE OF DIFFERENT DISEASES IN TULIPS

P.J. Muller

61. CONTROL OF SCLEROTIUM ROLFSII ON BULBOUS IRIS AND LILIES WITH IN-FURROW FUNGICIDE APPLICATIONS

Gary A. Chastagner | John M. Staley | K. Riley

62. DETECTION OF LATENT FUSARIUM OXYSPORUM IN GLADIOLUS CORMS

E.J.A. Roebroeck | N.P.A. Groen | J.J. Mes

63. NECK ROT DISEASE OF NARCISSUS CAUSED BY FUSARIUM OXYSPORUM F. SP. NARCISSI

C. A. Linfield

64. NEW TECHNOLOGIES FOR THE DETECTION AND IDENTIFICATION OF PATHOGENS IN BULBOUS CROPS WITH IMMUNOLOGICAL AND MOLECULAR HYBRIDIZATION TECHNIQUES.

65. HOT-WATER TREATMENT TO CONTROL COLLETOTRICHUM ACUTATUM ON CORMS OF ANEMONE CORONARIA

A.W. Doornik

66. HOT-WATER TREATMENT TOLERANCE IN GLADIOLUS CORMELS AND THEIR STATE OF DORMANCY

A. Cohen | A. Barzilay | H. Vigodsky-Haas

67. DECREASE IN VIABILITY OF COLLETOTRICHUM ACUTATUM IN CORMS OF ANEMONE CORONARIA DURING STORAGE

A.W. Doornik | E.M.C. Booden

68. ELECTRON MICROSCOPY OF VIRESCENT GLOXINIA PLANTS

M.G. Bellardi | A. Bertaccini

69. PRODUCTION FOR VIRUS FREEDOM OF SOME PRINCIPAL BULBOUS CROPS IN THE NETHERLANDS

C.J. Asjes

70. THE EFFICACY OF FOUR ANTIVIRAL COMPOUNDS IN THE ELIMINATION OF NARCISSUS VIRUSES DURING MERISTEM TIP CULTURE

S. Phillips

71. PHYSICAL, BIOLOGICAL AND CHEMICAL CONTROL OF TAENIOTHRIPS SIMPLEX IN GLADIOLUS

C.G.M. Conijn | N.P.A. Groen

72. CONTROL OF THE GLADIOLUS THRIPS, TAENIOTHRIPS SIMPLEX (MORISON), ON GLADIOLUS CROP

G. Labanowski | M. Mynett

73. EFFECTIVENESS OF PESTICIDES IN CONTROL OF NARCISSUS BULB FLY, MERODON EQUESTRIS (L.)

W. Bogatko | M. Mynett

74. EFFECT OF SEVERAL HERBICIDES ON GLADIOLUS PLANTS CULTIVATED FROM CORMS AND CORMELS

M. Mynett | M. Jagusz

75. HERBICIDE EVALUATION IN BULBOUS IRIS, NARCISSUS, AND TULIP

Stott W. Howard | Carl R. Libbey | Eric R. Hall

76. EFFECTS OF SHEETING WITH BLACK PLASTIC OR FLOODING OF SOILS ON THE CONTROL OF CYPERUS ESCULENTUS

A.Th.J. Koster | L.J. van der Meer

77. A DRIP-FREE MECHANICAL SYSTEM FOR APPLICATION OF CHEMICALS ON PLANTS

R.S. Bijl

78. EPILOGUE

Robert P. Doss