Articles
IN VITRO BULBLET PROPAGATION OF VIRUS-FREE DUTCH IRIS.
Article number
266_8
Pages
77 – 82
Language
Abstract
In vitro bulblet development of Dutch iris is optimized with the following media constituents and concentrations: 6% sucrose, full strength Murashige Skoog inorganics, 1231 uM NaH2PO4H2O, 555 uM myo inositol, 1.2 uM thiamine HCl, pH adjusted to 5.7, and 0.6–0.8% agar.
Once shoot multiplication and intermediate stages had been completed the shoots were transferred to the bulblet development medium.
The cultures were cooled 5° C for 4 weeks and then transferred to 30°C for 5–8 weeks.
The duration of heat period was limited by shoot sprouting; as the treatment was terminated when sprouting began.
Immediately following the 30°C period, the bulblets were again cooled at 5°C for 4 weeks before planting in the greenhouse.
Bulblets required 3 greenhouse cycles to develop sufficient bulb size for daughter bulb multiplication.
Once shoot multiplication and intermediate stages had been completed the shoots were transferred to the bulblet development medium.
The cultures were cooled 5° C for 4 weeks and then transferred to 30°C for 5–8 weeks.
The duration of heat period was limited by shoot sprouting; as the treatment was terminated when sprouting began.
Immediately following the 30°C period, the bulblets were again cooled at 5°C for 4 weeks before planting in the greenhouse.
Bulblets required 3 greenhouse cycles to develop sufficient bulb size for daughter bulb multiplication.
Publication
Authors
Wilbur C. Anderson, K. A. Mielke, P. N. Miller, T. Allen
Keywords
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