Articles
EFFECTS OF AUXIN AND CYTOKININ ON IN VITRO ORGANOGENESIS OF CINERARIA CHINENSIS L.
Article number
292_24
Pages
181 – 186
Language
Abstract
Explants from axillary buds of Cineraria chinensis were cultured on solid Murashige and Skoog medium with the addition of 6-benzylaminopurine (BA), kinetin, 2, 4-dichlorophenoxyacetic acid (2, 4-D), 3-indolebutyric acid (IBA), and naphthalene acetic acid (NAA). BA and kinetin tested at a rate of 0 to 20.0 mg/l mostly showed shoot formation, whereas BA at more than 0.1 mg/l produced abnormal shoot formation.
An optimal response was obtained using 0.1 mg/l kinetin, and its effects were better than that of BA as the latter caused vitrification of the plantlets.
IBA and NAA were used for root induction, where 0.5 mg/l NAA produced the best root formation. 2,4-D instead caused callus formation.
Plantlets were successfully transplanted and established in soil after two weeks of conditioning under strong light.
Cultures grew vigorously for 75 days.
An optimal response was obtained using 0.1 mg/l kinetin, and its effects were better than that of BA as the latter caused vitrification of the plantlets.
IBA and NAA were used for root induction, where 0.5 mg/l NAA produced the best root formation. 2,4-D instead caused callus formation.
Plantlets were successfully transplanted and established in soil after two weeks of conditioning under strong light.
Cultures grew vigorously for 75 days.
Authors
A.G.Abd. Karim, M. Hamidah
Keywords
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