Articles
CALLUS FORMATION FROM PROTOPLAST CULTURE OF PEACH
Article number
374_25
Pages
185 – 190
Language
Abstract
Embryogenic calli were obtained from the eight-week-old abortive embryo of peach (Prunus persica (L.) Batsh) cultivar "Sunagowase" cultured in MT basal medium.
Intact viable protoplasts were isolated from embryogenic calli after several subcultures.
After protoplast purification, a high protoplast yield (2.47 x 106/g-1FW) was obtained.
The purificatory protoplasts were incubated in PC-1 medium without growth hormone.
The first cell division was observed 5–7 days after the culture and a high plating density (about 26.4%) was obtained.
The colonies were transferred onto PC-2 medium 1 month later and calli were then observed.
It was indicated that the low melting point agarose (LMP) embedding culture is more favourable for peach protoplast compared with the thin liquid layer culture.
Intact viable protoplasts were isolated from embryogenic calli after several subcultures.
After protoplast purification, a high protoplast yield (2.47 x 106/g-1FW) was obtained.
The purificatory protoplasts were incubated in PC-1 medium without growth hormone.
The first cell division was observed 5–7 days after the culture and a high plating density (about 26.4%) was obtained.
The colonies were transferred onto PC-2 medium 1 month later and calli were then observed.
It was indicated that the low melting point agarose (LMP) embedding culture is more favourable for peach protoplast compared with the thin liquid layer culture.
Publication
Authors
G. Lin, D. Xiuxin, Z. Wencai
Keywords
peach, protoplast culture, callus regeneration
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