Articles
SEROLOGICAL DETECTION OF STRAWBERRY MILD YELLOW EDGE-ASSOCIATED VIRUS
Article number
385_12
Pages
98 – 104
Language
Abstract
Monoclonal and polyclonal antibodies were produced against the potexvirus associated with strawberry mild yellow edge disease.
For the polyclonal antiserum strawberry mild yellow edge associated virus (SMYEAV) was partially purified from Chenopodium quinoa co-infected with humulus japonicus ilarvirus.
For monoclonal antibody production mice were immunised with preparations of SMYEAV that were partially purified from aphid-inoculated Fragaria vesca UC-5 plants.
The monoclonal antibody EMA-48 was used in indirect double-antibody ELISA to trap antigen in combination with polyclonal detecting antibodies.
In this way SMYEAV was detected in samples from F. vesca infected with the homologous virus isolate, two isolates from Germany and from fourteen commercial strawberry plants from southern England.
The results were similar to those obtained using an all-polyclonal ELISA system and indicated the broad reactivity of EMA-48. During the summer, SMYEAV was detected in samples from the leaves, flowers and roots of commercial strawberry plants; strongest reactions were produced by samples from petals and from old leaves.
In host range studies, 38 species in 15 families were inoculated with SMYEAV by sap and/or aphids without finding a satisfactory propagation host, although the virus has been maintained for more than six consecutive passages in inoculated leaves of G foliosum.
For the polyclonal antiserum strawberry mild yellow edge associated virus (SMYEAV) was partially purified from Chenopodium quinoa co-infected with humulus japonicus ilarvirus.
For monoclonal antibody production mice were immunised with preparations of SMYEAV that were partially purified from aphid-inoculated Fragaria vesca UC-5 plants.
The monoclonal antibody EMA-48 was used in indirect double-antibody ELISA to trap antigen in combination with polyclonal detecting antibodies.
In this way SMYEAV was detected in samples from F. vesca infected with the homologous virus isolate, two isolates from Germany and from fourteen commercial strawberry plants from southern England.
The results were similar to those obtained using an all-polyclonal ELISA system and indicated the broad reactivity of EMA-48. During the summer, SMYEAV was detected in samples from the leaves, flowers and roots of commercial strawberry plants; strongest reactions were produced by samples from petals and from old leaves.
In host range studies, 38 species in 15 families were inoculated with SMYEAV by sap and/or aphids without finding a satisfactory propagation host, although the virus has been maintained for more than six consecutive passages in inoculated leaves of G foliosum.
Authors
A. Jawee, A.N. Adams
Keywords
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