Articles
UNEVEN DISTRIBUTION OF TOBACCO STREAK VIRUS IN STRAWBERRY PLANTLETS GROWN IN VITRO
Article number
385_17
Pages
122 – 125
Language
Abstract
Tobacco streak ilarvirus (TSV) is known to infect Fragaria spp. naturally.
In strawberry cultivars, infected plants remain symptomless, although a reduction in vigour and fruit yield was noted.
TSV-infected ‘Hood’ strawberry plantlets grown and proliferated in vitro on a nutrient medium were not uniformly infected.
Up to 30% of the progeny proliferated in vitro from known TSV-infected strawberry plants were found free of TSV, as measured by ELISA. In addition, the distribution of virus in the shoot and root systems of plantlets was uneven.
Root systems were more reliable for testing than leaf samples.
These results suggest that plants free of TSV can be recovered by in vitro cultivation and proliferation if enough stolon tips are established in vitro and that when testing plantlets for TSV by ELISA consideration should be given to the uneven distribution of virus.
In strawberry cultivars, infected plants remain symptomless, although a reduction in vigour and fruit yield was noted.
TSV-infected ‘Hood’ strawberry plantlets grown and proliferated in vitro on a nutrient medium were not uniformly infected.
Up to 30% of the progeny proliferated in vitro from known TSV-infected strawberry plants were found free of TSV, as measured by ELISA. In addition, the distribution of virus in the shoot and root systems of plantlets was uneven.
Root systems were more reliable for testing than leaf samples.
These results suggest that plants free of TSV can be recovered by in vitro cultivation and proliferation if enough stolon tips are established in vitro and that when testing plantlets for TSV by ELISA consideration should be given to the uneven distribution of virus.
Authors
S. Spiegel, Y. Tam, R.R. Martin, M. Ter Borg
Keywords
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