Articles
IN-STEM MOVEMENT, ISOLATION AND IDENTIFICATION OF TWO BACTERIA AND THEIR ANTIBIOTIC SENSITIVITY
Article number
41_16
Pages
183 – 198
Language
Abstract
Two bacterial species suspected of plugging the vascular system of cut flowers were followed with time in the snapdragon.
There was a relationship between the keeping quality of flowers and the presence of bacteria.
Water inoculated with a week-old culture of snapdragon stems and petals had the highest percentage of stem segments with bacteria.
Bacteria were most prevalent on the basal 5 to 25 mm segments and were also detected up to 150 m in some stems.
Isolation, characterization, and identification of the unknown stem bacteria through 30 different chemical and physiological tests confirmed the genera to be Flavobacterium and Pseudomonas.
In vitro experiments showed that both bacteria were highly sensitive to sulfacetamide, demethyl-chlortetracycline, and nalidixic acid.
There was a relationship between the keeping quality of flowers and the presence of bacteria.
Water inoculated with a week-old culture of snapdragon stems and petals had the highest percentage of stem segments with bacteria.
Bacteria were most prevalent on the basal 5 to 25 mm segments and were also detected up to 150 m in some stems.
Isolation, characterization, and identification of the unknown stem bacteria through 30 different chemical and physiological tests confirmed the genera to be Flavobacterium and Pseudomonas.
In vitro experiments showed that both bacteria were highly sensitive to sulfacetamide, demethyl-chlortetracycline, and nalidixic acid.
Authors
B. Dansereau, H.M. Vines
Keywords
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