Articles
A CONTRIBUTION TO THE STUDY OF SOME PROPERTIES OF THE SOUR CHERRY NECROTIC RING SPOT VIRUS
Article number
422_94
Pages
393 – 394
Language
Abstract
According to long-year observations the sour cherry necrotic ring spot belongs alongside with the plum pox virus respectively with other economically important diseases to the most extendend diseases of susceptible stone fruit species.
In the present work the properties of the sour cherry necrotic ring spot virus was studied on three isolates gained from Prunus cerasus L. in our ecological conditions.
The virus from diseased leaves and pollen was positively mechanically transmitted on the woody and herbaceous indicators.
In the juice of the infected cucumber plants the dilution end point ranged from 10-1 to 10-2, the termal point of inactivation from 48°C to 56 °C and the stability in vitro at ambient temperature from 1 to 2 days.
In the sedimentation profile two peaks were achieved.
The virus showed a typical absorption spectrum in the UV region.
The percentage of the ribonucleic acid was settled to 18% and the isoelectric point at pH 4,4. A special antiserum was prepared with antibodies 1:256.
In the present work the properties of the sour cherry necrotic ring spot virus was studied on three isolates gained from Prunus cerasus L. in our ecological conditions.
The virus from diseased leaves and pollen was positively mechanically transmitted on the woody and herbaceous indicators.
In the juice of the infected cucumber plants the dilution end point ranged from 10-1 to 10-2, the termal point of inactivation from 48°C to 56 °C and the stability in vitro at ambient temperature from 1 to 2 days.
In the sedimentation profile two peaks were achieved.
The virus showed a typical absorption spectrum in the UV region.
The percentage of the ribonucleic acid was settled to 18% and the isoelectric point at pH 4,4. A special antiserum was prepared with antibodies 1:256.
Authors
H. BAUMGARTNEROVÁ
Keywords
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