Articles

FERMENTATION OF OLIVE MILL WASTE-WATER BY LACTOBACILLUS PLANTARUM

Article number
474_153
Pages
735 – 740
Language
Abstract
A growth medium constituted by olive-mill waste – water (OMWW) and adjuvants such as tryptone and agar was used to grow Lactobacillus plantarum. A liquid/solid system was employed to improve the harvest of cells and to study the effect of each constituent on the growth, the metabolism and the fatty acid composition of the bacterial cells.
The presence of a nitrogen source, tryptone, was indispensable for the growth of L. plantarum and to limit the toxic effect of OMWW. An initial agar concentration of 1.5% was adequate for solidification of the medium allowing good diffusion of constituents.
The lactic acid production increased with the dose of tryptone and also of OMWW up to 49.4%. The cells incorporated oleic and linoleic acids present in OMWW and synthesized dihydrosterculic acid (C19Δcis-9,10) from oleic acid.
An optimal index was calculated from μmax, the maximum population and lactate production determined for each assay.

Publication
Authors
N. Rozès, J.F. Marques, C. Peres
Keywords
Olive mill waste – water, fermentation, Lactobacillus plantarum, fatty acid composition of bacterial cells
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