Home > VIII International Conference on Grape Genetics and Breeding > METHODOLOGICAL APPROACH FOR IMPROVED TRANSIENT GENE EXPRESSION IN VITIS VINIFERA L.

Articles

METHODOLOGICAL APPROACH FOR IMPROVED TRANSIENT GENE EXPRESSION IN VITIS VINIFERA L.

Article number

603_61

Pages

479 – 480

Language

English

Abstract

In order to improve transient gene expression levels, suspension cells were bombarded with plasmid DNA at three concentrations (0.1, 0.5 or 1 µg DNA per shot). Two particule sizes (1.0 or 1.6 µm) also were shot onto cells deposited on medium containing 20 or 60 g.l^-1 sucrose.
Results showed that high sucrose medium stimulated cell response while the larger particule size also induced more cells to GFP expression.
Experiments also indicated that higher level of expression could be obtained using half the control DNA amount

Publication

VIII International Conference on Grape Genetics and Breeding

Authors

PH. Chatelet, C. Brachet, G. Lopez, B. Khbaya, L. Torregrosa

Keywords

Vitis vinifera L., transient expression, biolistics, cell suspension

Full text

https://doi.org/10.17660/ActaHortic.2003.603.61

Online Articles (111)

2. MICROSATELLITE PROFILES AS A BASIS FOR INTELLECTUAL PROPERTY PROTECTION IN GRAPE

J. Ibañez | F.A. Van Eeuwijk

3. VITIS VINIFERA SSP. SILVESTRIS: THE STATE OF HEALT OF WILD ITALIAN AND SPANISH POPULATIONS ESTIMATED USING NUCLEAR AND CHLOROPLAST SSR ANALYSIS

S. Imazio | F. Grassi | A. Scienza | F. Sala | M. Labra

4. EU-PROJECT GENRES CT96 NO81: EUROPEAN VITIS DATABASE AND RESULTS REGARDING THE USE OF A COMMON SET OF MICROSATELLITE MARKERS

P. This | E Dettweiler

5. MOLECULAR MAPPING OF [REGENT X LEMBERGER] AND QTL-ANALYSIS OF AGRONOMIC TRAITS

6. COMPARATIVE MOLECULAR MAPPING IN SEGREGATING POPULATIONS OF GRAPEVINE

E. Zyprian | R. Eibach | R. Töpher

7. MAPPING XIPHINEMA INDEX RESISTANCE IN V. RUPESTRIS X M. ROTUNDIFOLIA HYBRIDS

M. Doucleff | Y. Jin | M.A. Walker

8. DEVELOPMENT OF GRAPEVINE RETROTRANSPOSON-BASED MOLECULAR MARKERS (S-SAP)

F. Pelsey | L. Schehrer | D. Merdinoglu

9. USE OF MOLECULAR MARKERS TO CHECK THE VARIETAL HOMOGENEITY OF BATCHES OF CUTTINGS

10. MOLECULAR ANALYSIS OF GRAPEVINE CULTIVARS INDIGENOUS IN THE CARPATHIAN BASIN

E. Kiss | A. Balogh | P. Kozma | T. Koncz | Z. Galli

11. STRATEGIES IN THE GENETIC SELECTION OF CLONES AND THE PRESERVATION OF GENETIC DIVERSITY WITHIN VARIETIES

H. Konrad | B. Lindner | E. Bleser | E.H. Rühl

12. DESCRIPTIVE ANALYSIS OF MERLOT AND CABERNET SAUVIGNON CLONAL WINES IN SOUTH AFRICA

C. Visser

13. AGRONOMIC AND ANALYTIC CLONAL VARIABILITY OF THE GRAPEVINE CULTIVAR CHASSELAS

D. Maigre

14. VINEYARD MANAGEMENT TO OPTIMIZE GRAPE QUALITY IN VIRUS-FREE CLONES OF VITIS VINIFERA L.

F. Mannini | L. Rolle | S. Guidoni

15. REPORT ON AN EFFICIENT SCHEME TO DEVELOP CERTIFIED CLONAL PROPAGATING MATERIAL IN AUSTRIA

H. Kaserer | W. Leonhardt | A. Grashsl | H. Gangl

16. FATE OF DNA DURING MUST FERMENTATION

S. Leopold | N. Uehlein | R. Kaldenhoff | A. Schartl

17. HIGH-DENSITY DNA ARRAYS FOR GRAPEVINE RESEARCH

L. Hausmann | W. Köglmeier | H. Düring | I. Salakhutdinov

18. SNP DETECTION AND GENOTYPING IN VITIS

C.L. Owens

19. GENETIC ANALYSIS OF A SEGREGATING POPULATION DERIVED BY A CROSS OF WELSCHRIESLING X SIRIUS

F. Regner | A. Fardossi | C. Eisenheld | I. Stierschneider

20. GENETIC ANALYSIS OF ROOT-KNOT NEMATODE RESISTANCE DERIVED FROM VITIS MUSTANGENSIS

P. Cousins | M. Lauver | L. Boyden

21. OCCURRENCE AND DISCRIMINATION OF SPONTANEOUS GRAPES NATIVE TO TUNISIA BY RAPD MARKERS

N. Zoghlami | A. Mliki | A. Ghorbel

22. A GENETIC VARIATION ANALYSIS OF V. VINIFERA CV. PINOT NOIR

A. Forneck | J. Konradi | R. Blaich

23. NARROW-SENSE HERITABILITY ESTIMATES FOR YIELD AND QUALITY CHARACTERISTICS IN CSIRO’S TABLE GRAPE BREEDING PROGRAM

X. Wei | P. Clingeleffer | S. Sykes

24. “SERBIA”, A NEW VERY EARLY TABLE GRAPEVINE VARIETY

L. Avramov | D. Tadijanovic | D. Zunic | K. Pavlovic | N. Gasic

25. BREEDING OF NEW SEEDLESS TABLE GRAPES IN ISRAEL CONVENTIONAL AND BIOTECHNOLOGICAL APPROACH

26. THE USE OF IN OVULO EMBRYO CULTURE FOR CROSS-BREEDING STUDIES OF EMPTY – SEEDED TABLE GRAPE CULTIVARS

H. Celik | A. Karli Ilbay

27. CYTO-EMBRYOLOGICAL ASPECTS OF SEEDLESSNESS IN VITIS VINIFERA L. AND EXPLOITING DNA RECOMBINANT TECHNOLOGY AS AN ADVANCED APPROACH FOR INTRODUCING SEEDLESSNESS INTO VINIFERA AND MUSCADINE GRAPES

V. Colova-Tsolova | J. Lu | A. Perl

28. CLUSTER AND BERRY CHARACTERISTICS OF FLAME SEEDLESS GRAPES UNDER DIFFERENT ENVIRONMENTAL CONDITIONS IN EGYPT

A. Hegazi | A. El Kader Sallam

29. INITIAL ANALYSIS OF VITIS VINIFERA ALCOHOL DEHYDROGENASE PROMOTER REGULATION

C. Tesniere | L. Torregrosa | C. Verries

30. ISOZYME AND DNA FINGERPRINTING CHARACTERIZATION OF TWO HUNGARIAN WINE GRAPE HYBRIDS AND THEIR PARENTS

M. Hajós-Novák | E. Hajdu

31. MOLECULAR, CHEMICAL AND MORPHOLOGICAL TOOLS TO EXPLORE VERTZAMI / MARZEMINO / BARZEMINO / BALSAMINA CULTIVAR GROUP

32. THE ARREST OF DEVELOPMENT OF USELESS REPRODUCTIVE ORGANS IN THE UNISEXUAL FLOWER OF VITIS VINIFERA SSP SILVESTRIS

A. Spada | E. Caporali | G. Marziani | O. Failla | A. Scienza

33. GENETIC RELATIONSHIPS AMONG GRAPE CULTIVARS FROM NORTH-WESTERN ITALY

A. Schneider | P. Boccacci | R. Botta

34. TOWARDS THE TRANSCRIPTOME OF GRAPEVINE (VITIS VINIFERA L.)

35. THE IDENTIFICATION OF ZINFANDEL ON THE DALMATIAN COAST OF CROATIA

36. VARIABILITY OF SOME AMPELOGRAPHIC PROPERTIES IN GRAPEVINE PROGENY CASTOR (B-7-2) X VRANAC

M. Milutinovic | V. Zivkovic | D. Zunic | D. Nikolic

37. THE VARIETAL IDENTIFICATION AND CHARACTERISATION WORK OF THE ‘ISTITUTO SPERIMENTALE PER LA VITICOLTURA’ IN THE PAST FIFTEEN YEARS

A. Costacurta | A. Calò | M. Crespan

38. CONTRIBUTION TO THE CLEARING UP OF SYNONYMIES IN SOME GROUPS OF ITALIAN GRAPEVINE CULTIVARS

39. GENETIC CHARACTERIZATION OF CLOSELY RELATED ROOTSTOCKS VARIETIES BASED ON AFLP AND SAMPL MARKERS

T. Wolf | J.-A. Cabezas | J.-M. Martinez-Zapater

40. GENETIC TRANSFORMATION OF VITIS VINIFERA TO IMPROVE DISEASE RESISTANCE

B.I. Reisch | J. Kikkert | J. Vidal | G.S. Ali | D. Gadoury

41. TRANSFORMATION OF VITIS VINIFERA L. CV NEBBIOLO WITH THE COAT PROTEIN GENE OF GRAPEVINE FANLEAF VIRUS (GFLV)

42. EVALUATION OF DIFFERENT GENE CONSTRUCTS FOR PRODUCTION OF RESISTANT GRAPEVINES AGAINST GRAPEVINE FANLEAF AND ARABIS MOSAIC VIRUSES

43. TRANSFER OF GRAPE FANLEAF VIRUS COAT PROTEIN GENE THROUGH HYBRIDIZATION WITH XIPHINEMA INDEX RESISTANT GENOTYPES TO OBTAIN ROOTSTOCKS RESISTANT TO VIRUS SPREAD

A. Bouquet | G. Marck | D. Pistagna | L. Torregrosa

44. INHERITANCE OF AROMA COMPOUNDS

R. Eibach | H. Hastrich | R. Töpfer

45. REALISATION OF NEW VARIABILITY BY MEANS OF INBREEDING IN SOME INDIGENOUS RED GRAPEVINE CULTIVAR OF VERONA (NORTH EAST ITALY)

G. Tamai | L. Brancadoro | O. Failla | A. Spinardi | A. Scienza

46. GRAPE BREEDING AT THE UNIVERSITY OF ARKANSAS: APPROACHING FORTY YEARS OF PROGRESS

J.R. Clark

47. REGULATION OF ANTHOCYANIN AND STILBENE PRODUCTION IN VITIS

J. Strommer | J. Coventry | A. Ali | D. Metcalf | H. Fischer

48. CHARACTERIZATION OF GRAPEVINE K⁺ CHANNELS AND THEIR IMPLICATION IN BERRY DEVELOPMENT AND QUALITY

R. Pratelli | L. Torregrosa | B. Lacombe | F. Gaymard

49. SUCCESS AND FAILURE: THE SPREAD OF NEW HUNGARIAN WINE-GRAPE VARIETIES IN HUNGARY OVER THE PAST 30 YEARS

J. Korbuly | G. Pernesz | I. Tóth

50. GRAPE BREEDING FOR RESISTANCE

P. Cindric | N. Korac | V. Kovac

51. UTILIZING THE COMPLETE PHYLLOXERA RESISTANCE OF VITIS CINEREA ARNOLD IN ROOTSTOCK BREEDING

J. Schmid | F. Manty | E.H. Rühl

52. GRAPEVINE IRON-CHLOROSIS OCCURRENCE AND STILBENE ROOT CONCENTRATION AS AFFECTED BY THE ROOTSTOCK AND ARBUSCULAR MYCORRHIZAL INFECTION

L. Bavaresco | S. Pezzutto | A. Fornaroli | F. Ferrari

53. SCREENING VITIS SPECIES FOR USE IN BREEDING MAGNESIUM TOLERANT ROOTSTOCKS

L. Kocsis | M.A. Walker

54. ISOLATION AND CHARACTERISATION OF RESISTANCE GENE ANALOGS (RGAS) IN GRAPE

G. Di Gaspero | G. Cipriani

55. PROGRESS IN UNDERSTANDING THE GENETICS OF RESISTANCE FROM THE GRAPE MUSCADINIA ROTUNDIFOLIA TO THE BACTERIAL PATHOGEN XYLELLA FASTIDIOSA

A.F. Krivanek | M.A. Walker

56. DEVELOPING RAPID EVALUATIONS FOR RESISTANCE TO XYLELLA FASTIDIOSA, THE CAUSAL AGENT OF PIERCE’S DISEASE

N. Buzkan | A.F. Krivanek | M.A. Walker

57. BREEDING FOR CROWN GALL RESISTANCE: TRADITIONAL AND MOLECULAR APPROACHES

T.J. Burr | L. Kovács | S. Süle | E. Szegedi

58. GENETIC ANALYSIS OF DOWNY MILDEW RESISTANCE DERIVED FROM MUSCADINIA ROTUNDIFOLIA

59. INHERITANCE OF RESISTANCE TO DOWNY MILDEW AND POWDERY MILDEW OF HYBRID FAMILY MUSCADINIA X V. VINIFERA X V. AMURENSIS X FRANCO-AMERICAN HYBRID

P. Kozma | T. Dula

60. GENETIC ANALYSIS OF THE GRAPEVINE CULTIVAR ‘PICOLIT’ BASED ON MICROSATELLITE AND AFLP MARKERS

L. Zulini | E. Fabro | E. Peterlunger

61. EVALUATION OF TOLERANCE TO PIERCE’S DISEASE AND BOTRYTIS IN TRANSGENIC PLANTS OF VITIS VINIFERA L. EXPRESSING THE PEAR PGIP GENE

C. Aguero | A. Dandekar | C. Meredith

62. METHODOLOGICAL APPROACH FOR IMPROVED TRANSIENT GENE EXPRESSION IN VITIS VINIFERA L.

PH. Chatelet | C. Brachet | G. Lopez | B. Khbaya | L. Torregrosa

63. GENETIC ENGINEERING OF GRAPE FOR RESISTANCE TO CROWN GALL

64. SELECTABLE MARKER SYSTEMS FOR GENETIC ENGINEERING OF GRAPEVINE

65. OPTIMIZATION OF CONDITIONS FOR REGENERATION AND GENETIC TRANSFORMATION OF ROOTSTOCK- AND SCION GRAPE VARIETIES

R. Olah | E. Szegedi | S. Ruthner | J. Korbuly

66. OVARY AS VALUABLE EXPLANT FOR SOMATIC EMBRYOGENESIS INDUCTION IN GRAPES (VITIS SPP.)

L. Martinelli | I. Gribaudo | M. Semenzato | V. Poletti

67. THE TWO VITIS DATABASES AS TOOLS FOR GERMPLASM MANAGEMENT VITIS INTERNATIONAL VARIETY CATALOGUE (HTTP://WWW.DAINET.DE/GENRES/IDB/VITIS) EUROPEAN VITIS DATABASE (HTTP://WWW.DAINET.DE/ECCDB/VITIS/)

E. Dettweiler | R. Eibach

68. GENOMICS TOOLS FOR MARKER ASSISTED SELECTION IN GRAPEVINE

69. SILENCING THE EXPRESSION OF AN AGROBACTERIUM VITIS ONCOGENE IN A MODEL PLANT

L. Kovács | S. Fekete | H. Hou | E. Segedi

70. QTL MAPPING FOR DISEASE RESISTANCE AND FRUIT QUALITY IN GRAPE

71. CHIMERISM AND GENETIC DIVERSITY WITHIN THE CULTIVAR GROUP OF PINOTS

S. Hocquigny | D. Merdinoglu | M.-C. Heloir | F. Pelsy

72. TWO -DIMENSIONAL ELECTROPHORETIC ANALYSIS APPLIED TO VITIS VINIFERA

J. Perugini | A. Schubert

73. GENETIC DIVERSITY IN VITIS VINIFERA SSP. SYLVESTRIS GMELIN FROM EUROPE, THE MIDDLE EAST AND NORTH AFRICA

A. Forneck | M.A. Walker | A. Schreiber | R. Blaich | F. Schumann

74. INVENTORY AND CHARACTERIZATION OF VITIS VINIFERA SSP. SILVESTRIS IN FRANCE

75. A NEW, HIGHLY ASSERTIVE SCAR MARKER POTENTIALLY USEFUL TO ASSIST SELECTION FOR SEEDLESSNESS IN TABLE GRAPE BREEDING

N. Mejía | P. Hinrichsen

76. BREEDING SEEDLESS GRAPES IN SOUTH AFRICA BY MEANS OF EMBRYO RESCUE

P. Burger | C.A. Gerber | A. Gerber | P.J.L. Ellis

77. STRUCTURE AND TRANSCRIPTION OF THREE CHALCONE SYNTHASE GENES OF GRAPEVINE (VITIS VINIFERA

N. Goto-Yamamoto | G.H. Wan | S. Kobayashi

78. APPLICATION OF MOLECULAR MARKERS FOR ANALYSIS OF PRESUMED SYNONYMS AND HOMONYMS WITHIN CROATIAN GRAPEVINE CULTIVARS

A. Vokurka | E. Maletic | A. Benjak | J. Karoglan-Kontic | I. Pejic

79. STUDY OF ORIGIN OF CULTIVAR ‘CSABA GYÖNGYE’ (PEARL OF CSABA)

80. ISOENZYMATIC CHARACTERISATION OF SOME GRAPEVINE CULTIVARS BRED IN BADACSONY

G. Györffyné Jahnke | J. Korbuly | J. Májer

81. CHARACTERIZATION OF GRAPE VARIETIES AND SPECIES BY RAPD MARKERS

82. GENETIC RESOURCES OF GRAPEVINE IN LEDNICE NA MORAVE

P. Pavloušek | E. Postbiegl

83. THE INFLUENCE OF MORPHOLOGICAL CHARACTERISTICS ON PRODUCTIVE PROPERTIES OF CULTIVAR STANUSINA

Z. Bozinovic | K. Beleski | K. Boskov

84. HERITABILITY OF FERTILITY OF THE KRATOSIJA VINE BIOTIPES

V. Maras | M. Milutinovic | L. Pejovic | A. Martinovic

85. MANAGEMENT AND CHARACTERIZATION OF A VITIS GERMPLASM BANK IN SPAIN

86. INTERACTION C.V. CABERNET FRANC/ TERROIR IN NORTHEASTERN ITALY

M. Stefanini | D. Porro | G. Colugnati | J.A. Voltolini

87. AMPELOGRAPHICAL DESCRIPTION OF NEW GRAPEVINE VARIETIES

P. Pavloušek

88. CLONAL SELECTION OF VERMENTINO GRAPEVINE VARIETY IN THE TUSCAN COASTAL AREA

89. SELECTION OF THE TABLE GRAPE VARIETY “PANNÓNIA KINCSE”

E. Hajdu | E Borbasne Sasköi

90. RESULTS OF GRAPE IMPROVEMENT BY CLONE SELECTION IN PÉCS

L. Diófási | G. Bíróné Toma | M. Khidhir Kinan

91. THE MOST RECENT RESULTS OF CLONE-SELECTION BREEDING IN EGER

R. Romenda

92. BREEDING STRATEGY FOR THE INTROGRESSION OF DISEASE RESISTANCE GENES INTO EUROPEAN GRAPEVINE

93. IMPROVEMENT OF AN INOCULUM PRODUCTION OF PLASMOPARA VITICOLA

S. Wiedemann-Merdinoglu | P. Coste | D. Merdinoglu

94. RESISTANT TO DISEASES VINE FORMS, OBTAINED BY INBREEDING

I. Todorov | G. Diakova | I. Tsvetkov

95. DISEASE-RESISTANT CULTIVARS AS A SOLUTION FOR ORGANIC VITICULTURE

P. Basler | H. Pfenninger

96. SUCCESS IN RESISTANCE BREEDING: “REGENT” AND ITS STEPS INTO THE MARKET

R. Eibach | R. Töpher

97. SELECTION OF PARAQUAT RESISTANT GRAPEVINE LINES IN BUD CULTURE AND PRELIMINARY INVESTIGATIONS

Z. Haydu

98. THE RESULTS OF THE CROSSBREEDING OF THE DEPARTMENT OF VITICULTURE

F. Benéyei | A. Lörincz | T. Gácsi | G. Lukáscy

99. TESTING OF NEW RED GRAPE VARIETIES AND CLONES IN VARIETY TRIALS

G. Pernesz | Z. Király-Véghely

100. WHITE WINE BREEDING AT KESZTHELY

L. Bakonyi

101. LA CRESCENT, A NEW COLD HARDY, HIGH QUALITY, WHITE WINE VARIETY

P.R. Hemstad | J.L. Luby

102. NARROW-SENSE HERITABILITY ESTIMATES FOR YIELD AND QUALITY CHARACTERISTICS IN CSIRO’S TABLE GRAPE BREEDING PROGRAM

X. Wei | P. Clingeleffer | S. Sykes

103. CHARACTERISTICS OF PROMISING GRAPEVINE HYBRIDS FROM DIFFERENT CROSSINGS

D. Nikolic | M. Milutinovic | V. Rakonjac | M. Fotiric

104. ROOTSTOCK BREEDING AT THE DEPARTMENT OF HORTICULTURE OF GEORGIKON FACULTY OF AGRICULTURE, UNIVERSITY OF VESZPRÉM

L. Bakonyi | K. Bakonyi | L. Kocsis | P. Kozma

105. LEVEL OF PHYLLOXERA RESISTANCE OF TWO NEW ROOTSTOCKS HYBRIDS: GEORGIKON 28 AND GEORGIKON 121

H. Tóth | L. Kocsis

106. NEWLY BRED VARIETIES OF PHYLLOXERA TOLERANT ROOTSTOCKS

A. Becker | A. Schartl | J.V. Herrmann

107. EFFECT OF ROOTSTOCK ON NUTRIENT TRANSPORT OF SCION VARIETIES UNDER ARID CONDITION

E. Miklós | E. Hajdu

108. SANITARY ASPECTS AND RESULTS OF THE HUNGARIAN GRAPE BREEDING

J. Lázár

109. CHARACTERIZATION OF FUNGUS RESISTANT GRAPE VARIETIES AND CANDIDATE VARIETIES IN PÉCS AR

A. Krizsics Cziskász | P. Kozma

110. RESULTS OF THE EXPERIMENTS FOR THE IMPROVEMENT OF THE FERTILISATION OF THE FUNCTIONAL FEMALE FLOWERED GRAPEVINE CULTIVAR ‘KÉKNYELÜ’

G. Györffyné Jahnke | J. Májer

111. POLLENIZER INFLUENCE ON BERRY AND SEED PROPERTIES IN GRAPEVINE CULTIVAR ‘BAGRINA’ (VITIS VINIFERA L.)

M. Fotiric | M. Mulitinovic | D. Nikolic | V. Rakonjac