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Articles

DEVELOPMENT OF GRAPEVINE RETROTRANSPOSON-BASED MOLECULAR MARKERS (S-SAP)

Article number
603_7
Pages
83 – 87
Language
English
Abstract
Derived from the multiplex marker system AFLP, we have developed for the grapevine genome, sequence-specific amplification polymorphism (S-SAP) markers, based on the location of retrotransposons relative to adjacent restriction endonuclease sites.
Insertional polymorphisms of three grapevine retrotransposons have been assessed within a set of 16 accessions of grapevine (species and varieties). Efficiency of the S-SAP fingerprinting has been compared to microsatellite markers.
S-SAPs enable to distinguish between all of the tested genotypes.
The topology of each resulting phenogram is unique, however, with obvious similarities.

Publication
Authors
F. Pelsey, L. Schehrer, D. Merdinoglu
Keywords
Vitis vinifera, genetic diversity, DNA fingerprinting, Ty1 copia-like retrotransposon.
Full text
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