Articles
MAPPING QTL ASSOCIATED WITH PHYTOPHTHORA ROOT ROT RESISTANCE IN CHILLI (CAPSICUM ANNUUM)
Article number
637_30
Pages
251 – 255
Language
English
Abstract
For mapping and characterizing the QTL related to Phytophthora capsici (PC) resistance, a molecular linkage map was constructed with RAPD and AFLP markers in an intra-specific BC1F1 population.
An introduced accession YCM334 is highly resistant to PC and a local variety, Taean, is susceptible to PC. A total of 43 BC1F1 plants from the cross between YCM334 and Taean were used for identification of DNA markers linked to PC resistance.
Twenty-eight linkage groups were constructed using 37 RAPD and 192 AFLP markers.
Average density of the map is approximately one marker for every 12.0 cM. Interval analysis detected two QTL associated with PC resistance.
The interval of AF191-AF300-RA5 on LG9 showed 3.15 LOD score for resistance.
This was also detected using the ANOVA model.
Another interval of AF138-AF316 on LG24 showed LOD 2.22, thus it is considered to have a minor effect on PC resistance.
ANOVA between DNA marker genotypes and resistance identified 7 markers.
Five of them were located on two linkage groups and the other 2 were not linked.
Three markers mapped on LG 9 had major effect on PC and explained 27.9% of phenotypic variance in a one-way ANOVA and 61.8% with AF56 marker in a two-way ANOVA. The AF56 marker showed negative effect on resistance when each plant had the YCM334 allele.
An introduced accession YCM334 is highly resistant to PC and a local variety, Taean, is susceptible to PC. A total of 43 BC1F1 plants from the cross between YCM334 and Taean were used for identification of DNA markers linked to PC resistance.
Twenty-eight linkage groups were constructed using 37 RAPD and 192 AFLP markers.
Average density of the map is approximately one marker for every 12.0 cM. Interval analysis detected two QTL associated with PC resistance.
The interval of AF191-AF300-RA5 on LG9 showed 3.15 LOD score for resistance.
This was also detected using the ANOVA model.
Another interval of AF138-AF316 on LG24 showed LOD 2.22, thus it is considered to have a minor effect on PC resistance.
ANOVA between DNA marker genotypes and resistance identified 7 markers.
Five of them were located on two linkage groups and the other 2 were not linked.
Three markers mapped on LG 9 had major effect on PC and explained 27.9% of phenotypic variance in a one-way ANOVA and 61.8% with AF56 marker in a two-way ANOVA. The AF56 marker showed negative effect on resistance when each plant had the YCM334 allele.
Authors
K.-T. Kim, H.S. Choi, Y. Chae, D.-G. Oh, B.D. Kim
Keywords
Capsicum annuum, Phytophthora capsici, DNA marker, QTL mapping
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