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Articles

COMPARISON OF DIFFERENT EXTRACTION PROCEDURES AND HPLC METHODS TO DETECT CROCINS IN SAFFRON

Article number

650_36

Pages

303 – 306

Language

English

Abstract

Isolation of crocins isomers has been studied in terms of extraction, focusing in the solvent variable (methanol or water) and its characterisation by two different chromatographic methods.
The use of methanol either on the extraction procedure or in the HPLC analysis produces crocins re-esterification.
Though, methanol may increase crocins extraction yield, but then it would be impossible to know the real ratio between crocins in saffron.

Publication

I International Symposium on Saffron Biology and Biotechnology

Authors

M. Carmona, A. Zalacaín, M.I. Rodríguez, M.R. Salinas, G.L. Alonso

Keywords

crocetin esters, methanol, re-esterification

Full text

https://doi.org/10.17660/ActaHortic.2004.650.36

Online Articles (60)

2. SAFFRON REPRODUCTIVE BIOLOGY

M. Grilli Caiola

3. FLOWER FORMATION IN THE SAFFRON CROCUS (CROCUS SATIVUS L.) THE ROLE OF TEMPERATURE

R.V. Molina | A. García-Luis | V. Coll | C. Ferrer | M. Valero | Y. Navarro | J.L. Guardiola

4. THE MORPHOLOGICAL STUDY OF AMYLOPLAST DISTRIBUTION IN CROCUS SATIVUS L. FIBROUS ROOTS

B. Behboodi | L. Samadi

5. DEVELOPMENT AND CONTRACTION OF CONTRACTILE ROOTS IN CROCUS SATIVUS

M. Khalesi | B. Behboodi | Ebrahimzadeh

6. COMPARATIVE MORPHOANATOMICAL STUDY OF THREE SPECIES PERTAINING TO THE CROCUS GENUS: C. NEVADENSIS, C. NUDIFLORUS AND C. SATIVUS DIFFERENTIATING CHARACTERISTICS

L. Pulido | S. Gattuso | M. Gattuso

7. CROCUS CARTWRIGHTIANUS ON THE ATTICA PENINSULA

N. Jacobsen | M. Ørgaard

8. BIOLOGY OF CROCUS OLIVIERI SUBSP. OLIVIERI

H. Vurdu | K. Güney | F.F. Çiçek

9. MOLECULAR BIOLOGICAL APPROACH OF THE SYSTEMATICS OF CROCUS SATIVUS L. AND ITS ALLIES

A.A. Zubor | G. Surányi | Z. Győri | G. Borbély | J. Prokisch

10. DEVELOPMENT OF MOLECULAR MARKERS FOR ORIGIN DETERMINATION IN SAFFRON

J. Pardo | J.-A. Fernández | L.G. Gómez

11. BIOSYNTHESIS OF CAROTENOIDS IN SAFFRON

A. Rubio | J.-A. Fernández | L.G. Gómez

12. EXPRESSION OF L-LACTATE DEHYDROGENASE ISOENZYMES DURING ROOT DEVELOPMENT IN CROCUS SATIVUS L. CORM

J. Keyhani | E. Keyhani | N. Sattarahmady

13. THE DISTINCTIVE FEATURES OF ASCORBATE PEROXIDASE IN DORMANT CROCUS SATIVUS L. CORM

L. Ghamsari | E. Keyhani

14. DETECTION AND KINETIC PROPERTIES OF ALCOHOL DEHYDROGENASE IN DORMANT CORM OF CROCUS SATIVUS L.

M. Hadizadeh | E. Keyhani

15. DEVELOPMENT AND GENE EXPRESSION IN SAFFRON CORMS

M. Álvarez-Ortí | L.G. Gómez | A. Rubio | J. Escribano | J. Pardo | F. Jiménez | J.-A. Fernández

16. STUDIES ON EXPRESSION OF GENES INVOLVED IN SOMATIC EMBRYOGENESIS AND STORAGE PROTEIN ACCUMULATION IN SAFFRON CROCUS (CROCUS SATIVUS L.)

M. Álvarez-Ortí | T. Schwarzacher | A. Rubio | S. Blázquez | A. Piqueras | J.-A. Fernández | P. Harrison

17. CHARACTERIZATION OF A CLASS I CHITINASE FROM SAFFRON (CROCUS SATIVUS L.)

R. Castillo | L.G. Gómez | J.-A. Fernández

18. INDIGENOUS KNOWLEDGE IN AGRICULTURE WITH PARTICULAR REFERENCE TO SAFFRON PRODUCTION IN IRAN

A.A. Koocheki

19. PRODUCTIVITY, GROWTH AND QUALITY ATTRIBUTES OF 10 IRANIAN SAFFRON ACCESSIONS UNDER CLIMATIC CONDITIONS OF CHAHAR-MAHAL BAKHTIARI, CENTRAL IRAN

P. Ehsanzadeh | A.A. Yadollahi | A.M.M. Maibodi

20. MORPHOLOGICAL CHARACTERISTICS OF GREEK SAFFRON STIGMAS FROM KOZANI REGION

T. Mitsopoulou | M.Z. Tsimidou

21. EXPERIMENTAL FINDINGS OF PRODUCTION AND ECHO PHYSIOLOGICAL ASPECTS OF SAFFRON (CROCUS SATIVUS L.)

A. Mollafilabi

22. THE EFFECT OF NITROGEN FERTILIZERS (UREA, SULFUR COATED UREA) WITH MANURE ON THE SAFFRON YIELD

S. Rezaian | M. Forouhar

23. INFLUENCE OF FOLIAR FERTILIZATION ON YIELD OF SAFFRON (CROCUS SATIVUS L.)

M. Hosseini | B. Sadeghiand | S.A. Aghamiri

24. EVALUATION OF SAFFRON (CROCUS SATIVUS L.) YIELD COMPONENTS

A. Mollafilabi

25. MODELING SAFFRON FLOWERING TIME ACROSS A TEMPERATURE GRADIENT

M.A. Behdani | M. Nassiri | A.A. Koocheki

26. EXTENDING THE HARVEST PERIOD OF SAFFRON

R.V. Molina | A. García-Luis | M. Valero | Y. Navarro | J.L. Guardiola

27. CULTIVATION TECHNIQUES, MORPHOLOGY AND ENZYMATIC PROPERTIES OF CROCUS SATIVUS L.

E. Keyhani | J. Keyhani | M. Hadizadeh | L. Ghamsari | F. Attar

28. MODALITY OF THE CONTRACTILE ROOT FORMATION IN CULTIVATED SAFFRON, IN FIELD CONDITION AND IN TISSUE CULTURE

M. Khalesi | B. Behboodi | H. Ebrahimzadeh

29. PLANTLET REGENERATION VIA SOMATIC EMBRYOGENESIS IN FOUR SPECIES OF CROCUS

R. Karamian

30. SOMATIC EMBRYOGENESIS IN SAFFRON (CROCUS SATIVUS L.): MORPHOLOGICAL DIFFERENTIATION AND THE ROLE OF THE ANTIOXIDANT ENZYMATIC SYSTEM

S. Blázquez | E. Olmos | J.A. Hernández | E. Hellín | J.-A. Fernández | A. Piqueras

31. SOMATIC EMBRYOGENESIS IN SAFFRON: OPTIMISATION THROUGH TEMPORARY IMMERSION AND POLYAMINE METABOLISM

S. Blázquez | A. Piqueras | M.D. Serna | J.L. Casas | J.-A. Fernández

32. COLCHICINE INDUCED VARIABILITY IN SAFFRON

G. Zaffar | S.A. Wani | T. Anjum | N.A. Zeerak

33. INDUCED MUTAGENIC VARIABILITY IN SAFFRON (CROCUS SATIVUS L.)

I.A. Khan

34. ROOM TABLE: AGRONOMICAL AND BIOTECHNOLOGICAL APPROACHES FOR SAFFRON IMPROVEMENT

H. Vurdu

35. SAFFRON: ON THE CONTRIBUTION OF COLORANTS TO ITS COMMERCIAL QUALITY

M.Z. Tsimidou

36. A RAPID PROCEDURE FOR THE EVALUATION OF SAFFRON COLOURING STRENGTH USING TRISTIMULUS COLORIMETRY

L. Cuko | T. Mitsopoulou | M.Z. Tsimidou

37. COMPARISON OF DIFFERENT EXTRACTION PROCEDURES AND HPLC METHODS TO DETECT CROCINS IN SAFFRON

M. Carmona | A. Zalacaín | M.I. Rodríguez | M.R. Salinas | G.L. Alonso

38. IMPROVED CONDITIONS TO EXTRACT EXOGENOUS YELLOW WATER-SOLUBLE COLORANTS ADDED TO SAFFRON AVOIDING CROCETIN ESTERS INTERFERENCES

A. Zalacaín | I. Blázquez | E.M. Díaz-Plaza | M. Carmona | G.L. Alonso

39. KINETIC STUDY OF THE PHOTODECOLORATION OF THE SAFFRON

M. del Rosario Haro | D. Escolar | C. Bueno | J. Ayuso

40. CHEMICAL COMPOSITION OF SAFFRON (CROCUS SATIVUS L.) FROM FOUR COUNTRIES

H. Caballero-Ortega | R. Pereda-Miranda | L. Riverón-Negrete | J.M. Hernández | M. Medécigo-Ríos | A. Castillo-Villanueva | F.I. Abdullaev

41. FT-NIR SPECTROMETRY APPROACH FOR DETERMINING SAFFRON ORIGIN

A. Zalacaín | E.M. Díaz-Plaza | I. Blázquez | M. Carmona | G.L. Alonso

42. DETECTION OF ARTIFICIAL RED COLORANTS IN SAFFRON USING UV-VIS SPECTROMETRY AND TRISTIMULUS COLORIMETRY

S.A. Ordoudi | M.Z. Tsimidou

43. DECONTAMINATION OF SAFFRON (CROCUS SATIVUS L.) BY ELECTRON BEAM IRRADIATION

H.B. Ghoddusi | B. Glatz

44. EVALUATION OF QUALITY CHARACTERISTICS AND MICROBIAL CONTAMINATION OF SAFFRON SAMPLES DRIED BY MICROWAVE

M.H. Nejad

45. SAFFRON BY-PRODUCTS INTEGRATED VALORISATION USING AGRORESOURCE REFINING CONCEPT (ARC)

M. Bergoin | C. Raynaud | G. Vilarem | J.-M. Bessière | T. Talou

46. EXTRACTION AND EVALUATION OF SAFFRON OLEORESIN

Q. Rajabzadeh | R. Malekzadeh | A. Sharif

47. THE APPLICATION OF CROCIN AND SAFFRON ETHANOL-EXTRACTABLE COMPONENTS IN FORMULATION OF HEALTH CARE AND BEAUTY CARE PRODUCTS

H. Fekrat

48. NEW SAFFRON PRODUCTS AND FOOD TECHNOLOGY

A. Shariati-Moghaddam

49. A NEW LOOK AT SAFFRON: MISTAKEN BELIEFS

M. Carmona | G.L. Alonso

50. HOW CAN ANTHROPOLOGY CONTRIBUTE TO THE EXPANSION OF SAFFRON PRODUCTION?

S. Helfer

51. SAFFRON NEEDS MARKETING

J. Rohmeder

52. ROOM TABLE: INDUSTRIAL PERSPECTIVES FOR SAFFRON

J.-M. Thiercelin

53. THE THERAPEUTIC AND PROPHYLACTIC EFFECTS OF CROCUS SATIVUS L. (SAFFRON) IN SENILE DEMENTIA

H. Saito

54. PREVENTION OF PC-12 CELL DEATH BY CROCIN VIA SPHINGOMYELINASE-CERAMIDE SIGNALING BY INCREASE OF GLUTATHIONE BIOSYNTHESIS

T. Ochiai | S. Soeda | S. Ohno | H. Shimeno | H. Tanaka | Y. Shoyama | J. Molnar | L. Berek | T. Bartok

55. ANTIDEPRESSANT EFFECT OF CROCUS SATIVUS L. STIGMA EXTRACTS AND THEIR CONSTITUENTS, CROCIN AND SAFRANAL, IN MICE

H. Hosseinzadeh | G. Karimi | M. Niapoor

56. CHEMICAL ANALYSIS AND ANTITUMOR ACTIVITY OF NATURAL AND SEMI-NATURAL CAROTENOIDS OF SAFFRON

P.A. Tarantilis | M.G. Polissiou

57. EFFECT OF SAFFRON ON THE VIABILITY OF NORMAL AND MALIGNANT HUMAN CELLS IN VITRO

F. Trujillo-Jiménez | P. García-López | A. Garcí-Carrancá | F.I. Abdullaev

58. EVALUATION OF THE MUTAGENIC AND ANTIMUTAGENIC ACTIVITIES OF SAFFRON

J.M. Hernández | F.I. Abdullaev | H. Caballero-Ortega | L. Riverón-Negrete | I. Pérez-López | M. Medécigo-Ríos

59. ADDITIVE INHIBITORY EFFECT OF SAFFRON IN COMBINATION WITH SODIUM SELENITE ON TUMOR CELLS

L. Riverón-Negrete | R. Camacho-Carranza | H. Caballero-Ortega | J.M. Hernández | M. Medécigo-Ríos | A. Castillo-Villanueva | I. Pérez-López | J.J. Espinosa-Aguirre | F. Abdullaev

60. PRESENCE OF BIOACTIVE GLYCOCONJUGATES ON DIFFERENT STAGES OF SAFFRON CORM

A. Rubio | P. Fernández-Nohales | G. Gerwig | J. Escribano | J.P. Kamerling | J.-A. Fernández