Articles
SOME PROPERTIES OF A YELLOW MOSAIC VIRUS ISOLATED FROM BRAMBLE
Article number
66_10
Pages
79 – 84
Language
Abstract
A sap-transmitted virus found associated with a yellow mosaic disease of a wild trailing blackberry (Rubus rigidus) has a restricted host range infecting a few species in Amaranthaceae, Chenopodiaceae and Solanaceae.
The virus remained infective in Chenopodium murale sap after dilution to 10-3, or after 10 min at 50°C (but not 55°C), 8 but not 16 days at 20°C. Infectivity was lost at pH 6.0 and after prolonged freezing (3 weeks) but was only slightly decreased after 24 ha at -20°C. It is seed-borne in C. murale (86–100%), showing systemic symptoms in progeny seedlings and induced typical yellow mosaic disease symptoms 2–3 months after being returned to Fragaria vesca.
Partially purified virus preparations obtained by differential centrifugation and sucrose density gradient centrifugation, following chloroform clarification, yielded flexuous filamentous particles c. 730 nm.
The virus remained infective in Chenopodium murale sap after dilution to 10-3, or after 10 min at 50°C (but not 55°C), 8 but not 16 days at 20°C. Infectivity was lost at pH 6.0 and after prolonged freezing (3 weeks) but was only slightly decreased after 24 ha at -20°C. It is seed-borne in C. murale (86–100%), showing systemic symptoms in progeny seedlings and induced typical yellow mosaic disease symptoms 2–3 months after being returned to Fragaria vesca.
Partially purified virus preparations obtained by differential centrifugation and sucrose density gradient centrifugation, following chloroform clarification, yielded flexuous filamentous particles c. 730 nm.
Authors
D.J. Engelbrecht
Keywords
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