Articles
SEROLOGICAL DETECTION OF VIRUSES IN RUBUS SAP
Article number
66_7
Pages
53 – 62
Language
Abstract
Three sap-transmissible viruses, raspberry bushy dwarf virus (RBDV), tobacco streak virus (TSV), and tomato ringspot virus (TmRSV), were detected in sap of several cultivated Rubus species by agar gel serological tests.
Sap from Rubus plants that were known to be virus infected was extracted in various solutions that gave positive serological tests.
Extracting buffers optimal for seroassay were not always optimal for bioassay, and vice versa.
The most consistent and sharply delineated precipitin lines were obtained using 0.1 M Tricine, pH 8, plus 4% polyethylene glycol, MW 6000 (PEG-Tricine buffer) in the maceration of young infected Rubus shoots in a weight:volume ratio of 1:5. Although precipitin lines often occurred within a few hours, final readings were made at 5–7 days.
Healthy Rubus sap extracted in PEG-Tricine buffer, buffer alone, and normal sera were used as controls.
The PEG-Tricine seroassay was 95% reliable from known TSV-infected black raspberries, and 40% reliable from known TmRSV-infected red raspberries.
Seroassays of coded samples of healthy and TSV-infected red raspberry were 100% accurate.
Sap from Rubus plants that were known to be virus infected was extracted in various solutions that gave positive serological tests.
Extracting buffers optimal for seroassay were not always optimal for bioassay, and vice versa.
The most consistent and sharply delineated precipitin lines were obtained using 0.1 M Tricine, pH 8, plus 4% polyethylene glycol, MW 6000 (PEG-Tricine buffer) in the maceration of young infected Rubus shoots in a weight:volume ratio of 1:5. Although precipitin lines often occurred within a few hours, final readings were made at 5–7 days.
Healthy Rubus sap extracted in PEG-Tricine buffer, buffer alone, and normal sera were used as controls.
The PEG-Tricine seroassay was 95% reliable from known TSV-infected black raspberries, and 40% reliable from known TmRSV-infected red raspberries.
Seroassays of coded samples of healthy and TSV-infected red raspberry were 100% accurate.
Young Rubus shoots infected with TSV, that were either forced in the greenhouse or obtained from the field, were successfully seroassayed from February-June.
The seroassays became progressively less reliable during July and August, and were only successful during late summer and autumn with virus preparations from Rubus which had been partially purified.
TSV was detected in dormant winter Rubus buds and in Rubus pollen by seroassay.
Authors
R.H. Converse
Keywords
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